Using Fura-2AM microfluorimetry, it was shown for the first time that phospholipase A ₂ inhibitors 4-bromophenacyl bromide and glucocorticosteroids prednisolone and dexamethasone attenuate Ca ²⁺ responses induced by neuroleptic trifluoperazine in macrophages. The results suggest the involvement of phospholipase A ₂ and arachidonic acid metabolism cascade in the effect of trifluoperazine on intracellular Ca ²⁺ concentration in macrophages.