Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

Standard

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks. / Beliavskaia, Alexandra; Hönig, Vaclav; Erhart, Jan; Vyhlidalova, Tereza; Palus, Martin; Cerny, Jiri; Kozlova, Irina; Ruzek, Daniel; Palomar, Ana M.; Bell-Sakyi, Lesley.

In: Frontiers in Veterinary Science, Vol. 8, 659786, 26.03.2021, p. 257.

Research output: Contribution to journal › Article › peer-review

Harvard

Beliavskaia, A, Hönig, V, Erhart, J, Vyhlidalova, T, Palus, M, Cerny, J, Kozlova, I, Ruzek, D, Palomar, AM & Bell-Sakyi, L 2021, 'Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks', Frontiers in Veterinary Science, vol. 8, 659786, pp. 257. https://doi.org/10.3389/fvets.2021.659786

APA

Beliavskaia, A., Hönig, V., Erhart, J., Vyhlidalova, T., Palus, M., Cerny, J., Kozlova, I., Ruzek, D., Palomar, A. M., & Bell-Sakyi, L. (2021). Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks. Frontiers in Veterinary Science, 8, 257. [659786]. https://doi.org/10.3389/fvets.2021.659786

Vancouver

Beliavskaia A, Hönig V, Erhart J, Vyhlidalova T, Palus M, Cerny J et al. Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks. Frontiers in Veterinary Science. 2021 Mar 26;8:257. 659786. https://doi.org/10.3389/fvets.2021.659786

Author

Beliavskaia, Alexandra ; Hönig, Vaclav ; Erhart, Jan ; Vyhlidalova, Tereza ; Palus, Martin ; Cerny, Jiri ; Kozlova, Irina ; Ruzek, Daniel ; Palomar, Ana M. ; Bell-Sakyi, Lesley. / Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks. In: Frontiers in Veterinary Science. 2021 ; Vol. 8. pp. 257.

BibTeX

@article{6bb4d9b045a34699ae486cb4827da1e8,

title = "Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks",

abstract = "Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.",

keywords = "endosymbiont, Ixodes persulcatus, Spiroplasma, tick cell line, tick colony",

author = "Alexandra Beliavskaia and Vaclav H{\"o}nig and Jan Erhart and Tereza Vyhlidalova and Martin Palus and Jiri Cerny and Irina Kozlova and Daniel Ruzek and Palomar, {Ana M.} and Lesley Bell-Sakyi",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Beliavskaia, H{\"o}nig, Erhart, Vyhlidalova, Palus, Cerny, Kozlova, Ruzek, Palomar and Bell-Sakyi.",

year = "2021",

month = mar,

day = "26",

doi = "10.3389/fvets.2021.659786",

language = "English",

volume = "8",

pages = "257",

journal = "Frontiers in Veterinary Science",

issn = "2297-1769",

publisher = "KOREAN SOC SEXUAL MEDICINE & ANDROLOGY",

}

RIS

TY - JOUR

T1 - Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

AU - Beliavskaia, Alexandra

AU - Hönig, Vaclav

AU - Erhart, Jan

AU - Vyhlidalova, Tereza

AU - Palus, Martin

AU - Cerny, Jiri

AU - Kozlova, Irina

AU - Ruzek, Daniel

AU - Palomar, Ana M.

AU - Bell-Sakyi, Lesley

PY - 2021/3/26

Y1 - 2021/3/26

N2 - Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

AB - Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

KW - endosymbiont

KW - Ixodes persulcatus

KW - Spiroplasma

KW - tick cell line

KW - tick colony

UR - http://www.scopus.com/inward/record.url?scp=85103878204&partnerID=8YFLogxK

U2 - 10.3389/fvets.2021.659786

DO - 10.3389/fvets.2021.659786

M3 - Article

AN - SCOPUS:85103878204

VL - 8

SP - 257

JO - Frontiers in Veterinary Science

JF - Frontiers in Veterinary Science

SN - 2297-1769

M1 - 659786

ER -

ID: 88620100