Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks. / Beliavskaia, Alexandra; Hönig, Vaclav; Erhart, Jan; Vyhlidalova, Tereza; Palus, Martin; Cerny, Jiri; Kozlova, Irina; Ruzek, Daniel; Palomar, Ana M.; Bell-Sakyi, Lesley.
в: Frontiers in Veterinary Science, Том 8, 659786, 26.03.2021, стр. 257.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks
AU - Beliavskaia, Alexandra
AU - Hönig, Vaclav
AU - Erhart, Jan
AU - Vyhlidalova, Tereza
AU - Palus, Martin
AU - Cerny, Jiri
AU - Kozlova, Irina
AU - Ruzek, Daniel
AU - Palomar, Ana M.
AU - Bell-Sakyi, Lesley
N1 - Publisher Copyright: © Copyright © 2021 Beliavskaia, Hönig, Erhart, Vyhlidalova, Palus, Cerny, Kozlova, Ruzek, Palomar and Bell-Sakyi.
PY - 2021/3/26
Y1 - 2021/3/26
N2 - Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.
AB - Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.
KW - endosymbiont
KW - Ixodes persulcatus
KW - Spiroplasma
KW - tick cell line
KW - tick colony
UR - http://www.scopus.com/inward/record.url?scp=85103878204&partnerID=8YFLogxK
U2 - 10.3389/fvets.2021.659786
DO - 10.3389/fvets.2021.659786
M3 - Article
AN - SCOPUS:85103878204
VL - 8
SP - 257
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
SN - 2297-1769
M1 - 659786
ER -
ID: 88620100