Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation

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Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation. / Slyusarenko, Maria ; Nikiforova, Nadezhda; Sidina, Elena; Nazarova, Inga; Egorov, Vladimir; Garmay, Yuri; Merdalimova, Anastasiia; Yevlampieva, Natalia; Gorin, Dmitry; Malek, Anastasia.

In: Polymers, Vol. 13, No. 3, 458, 31.01.2021, p. 1-16.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{d18a157f1668408196a1bfd7bdcd4e0a,

title = "Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation",

abstract = "The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.",

keywords = "Dextran, Diagnostic, Extracellular nanovesicles, Plasma, Polyethylene glycol, Two‐-phase polymer system, two-phase polymer system, TRYPTOPHAN, polyethylene glycol, FRACTIONATION, PARTICLES, VESICLES, IDENTIFICATION, extracellular nanovesicles, POLY(ETHYLENE GLYCOL), dextran, diagnostic, plasma",

author = "Maria Slyusarenko and Nadezhda Nikiforova and Elena Sidina and Inga Nazarova and Vladimir Egorov and Yuri Garmay and Anastasiia Merdalimova and Natalia Yevlampieva and Dmitry Gorin and Anastasia Malek",

note = "Funding Information: Funding: This research was supported by Ministry of Health of the Russian Federation (project № AAAA‐A18‐118032890186‐5). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = jan,

day = "31",

doi = "10.3390/polym13030458",

language = "English",

volume = "13",

pages = "1--16",

journal = "Polymers",

issn = "2073-4360",

publisher = "MDPI AG",

number = "3",

}

RIS

TY - JOUR

T1 - Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation

AU - Slyusarenko, Maria

AU - Nikiforova, Nadezhda

AU - Sidina, Elena

AU - Nazarova, Inga

AU - Egorov, Vladimir

AU - Garmay, Yuri

AU - Merdalimova, Anastasiia

AU - Yevlampieva, Natalia

AU - Gorin, Dmitry

AU - Malek, Anastasia

N1 - Funding Information: Funding: This research was supported by Ministry of Health of the Russian Federation (project № AAAA‐A18‐118032890186‐5). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/1/31

Y1 - 2021/1/31

N2 - The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.

AB - The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.

KW - Dextran

KW - Diagnostic

KW - Extracellular nanovesicles

KW - Plasma

KW - Polyethylene glycol

KW - Two‐-phase polymer system

KW - two-phase polymer system

KW - TRYPTOPHAN

KW - polyethylene glycol

KW - FRACTIONATION

KW - PARTICLES

KW - VESICLES

KW - IDENTIFICATION

KW - extracellular nanovesicles

KW - POLY(ETHYLENE GLYCOL)

KW - dextran

KW - diagnostic

KW - plasma

UR - http://www.scopus.com/inward/record.url?scp=85100503237&partnerID=8YFLogxK

U2 - 10.3390/polym13030458

DO - 10.3390/polym13030458

M3 - Article

AN - SCOPUS:85100503237

VL - 13

SP - 1

EP - 16

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 3

M1 - 458

ER -

ID: 88220582