Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation. / Slyusarenko, Maria; Nikiforova, Nadezhda; Sidina, Elena; Nazarova, Inga; Egorov, Vladimir; Garmay, Yuri; Merdalimova, Anastasiia; Yevlampieva, Natalia; Gorin, Dmitry; Malek, Anastasia.
в: Polymers, Том 13, № 3, 458, 31.01.2021, стр. 1-16.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Formation and evaluation of a two-phase polymer system in human plasma as a method for extracellular nanovesicle isolation
AU - Slyusarenko, Maria
AU - Nikiforova, Nadezhda
AU - Sidina, Elena
AU - Nazarova, Inga
AU - Egorov, Vladimir
AU - Garmay, Yuri
AU - Merdalimova, Anastasiia
AU - Yevlampieva, Natalia
AU - Gorin, Dmitry
AU - Malek, Anastasia
N1 - Funding Information: Funding: This research was supported by Ministry of Health of the Russian Federation (project № AAAA‐A18‐118032890186‐5). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/1/31
Y1 - 2021/1/31
N2 - The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.
AB - The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.
KW - Dextran
KW - Diagnostic
KW - Extracellular nanovesicles
KW - Plasma
KW - Polyethylene glycol
KW - Two‐-phase polymer system
KW - two-phase polymer system
KW - TRYPTOPHAN
KW - polyethylene glycol
KW - FRACTIONATION
KW - PARTICLES
KW - VESICLES
KW - IDENTIFICATION
KW - extracellular nanovesicles
KW - POLY(ETHYLENE GLYCOL)
KW - dextran
KW - diagnostic
KW - plasma
UR - http://www.scopus.com/inward/record.url?scp=85100503237&partnerID=8YFLogxK
U2 - 10.3390/polym13030458
DO - 10.3390/polym13030458
M3 - Article
AN - SCOPUS:85100503237
VL - 13
SP - 1
EP - 16
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 3
M1 - 458
ER -
ID: 88220582