Research output: Contribution to journal › Article › peer-review
The aim of the study was to explore the polyethylene glycol–dextran two‐-phase polymer system formed in human plasma to isolate the exosome‐-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separa-tion. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectros-copy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In con-clusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT‐-qPCR), re-spectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.
Original language | English |
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Article number | 458 |
Pages (from-to) | 1-16 |
Number of pages | 15 |
Journal | Polymers |
Volume | 13 |
Issue number | 3 |
DOIs | |
State | Published - 31 Jan 2021 |
ID: 88220582