TY - CONF

T1 - Variability of Antimicrobial and Antifungal effect of LACTOBACILLUS PLANTARUM and LACTOBACILLUS ACIDOPHILUS

AU - Рыбальченко, Оксана Владимировна

AU - Орлова, Ольга Геннадьевна

AU - Капустина, Валентина Викторовна

N1 - Conference code: 11

PY - 2021

Y1 - 2021

N2 - An essential point in the prevention and complex bacteriotherapy of dysbiosisis the level and spectrum of antagonistic activity of probiotic bacteria.Aim:To reveal the nature of the relationship between industrial strainsof lactobacilli (LB) and opportunistic microorganisms (UPM) at theultrastructural level.Materials and methods:Industrial strains Lactobacillus plantarum 8RA-3 and L. acidophilus D75.Clinical isolates: 8 strains of S. aureus producing ά-hemolysin, 20 strainsof E. coli Hly +, 12 strains of C. albicans were detected using transmissionelectron microscopy on a JEM-100C (JEOL, Japan).Results:With the manifestation of antagonistic activity of LB in relation to UPBof various types, significant changes were found in all interacting cells.Thus, extensive invaginations of intracytoplasmic membrane structuresappeared in LB cells, the formation of which indicated the activation ofmetabolic processes in them.Electron microscopic examination of co-grown cultures of LB with UPB andC. albicans in places of close cell contact revealed significant destructivechanges in the cells of LB themselves. The main differences were in thenature of the destruction of cell walls by the type of desquamation of smalllayer-by-layer fragments of peptidoglycan layers. Along with destructivechanges in the cell wall, a specific change in the ultrafine structure ofthe protein-ribosomal complex of the cytoplasm of lactobacilli was noted.Conclusion:Ultrastructural changes revealed during the joint cultivation of LB with S.aureus, E. coli Hly +, and C. albicans testified to the strict specificity of theinteraction of these microorganisms.

AB - An essential point in the prevention and complex bacteriotherapy of dysbiosisis the level and spectrum of antagonistic activity of probiotic bacteria.Aim:To reveal the nature of the relationship between industrial strainsof lactobacilli (LB) and opportunistic microorganisms (UPM) at theultrastructural level.Materials and methods:Industrial strains Lactobacillus plantarum 8RA-3 and L. acidophilus D75.Clinical isolates: 8 strains of S. aureus producing ά-hemolysin, 20 strainsof E. coli Hly +, 12 strains of C. albicans were detected using transmissionelectron microscopy on a JEM-100C (JEOL, Japan).Results:With the manifestation of antagonistic activity of LB in relation to UPBof various types, significant changes were found in all interacting cells.Thus, extensive invaginations of intracytoplasmic membrane structuresappeared in LB cells, the formation of which indicated the activation ofmetabolic processes in them.Electron microscopic examination of co-grown cultures of LB with UPB andC. albicans in places of close cell contact revealed significant destructivechanges in the cells of LB themselves. The main differences were in thenature of the destruction of cell walls by the type of desquamation of smalllayer-by-layer fragments of peptidoglycan layers. Along with destructivechanges in the cell wall, a specific change in the ultrafine structure ofthe protein-ribosomal complex of the cytoplasm of lactobacilli was noted.Conclusion:Ultrastructural changes revealed during the joint cultivation of LB with S.aureus, E. coli Hly +, and C. albicans testified to the strict specificity of theinteraction of these microorganisms.

M3 - Paper

SP - 49

Y2 - 12 September 2021 through 14 September 2021

ER -