An essential point in the prevention and complex bacteriotherapy of dysbiosis
is the level and spectrum of antagonistic activity of probiotic bacteria.
Aim:
To reveal the nature of the relationship between industrial strains
of lactobacilli (LB) and opportunistic microorganisms (UPM) at the
ultrastructural level.
Materials and methods:
Industrial strains Lactobacillus plantarum 8RA-3 and L. acidophilus D75.
Clinical isolates: 8 strains of S. aureus producing ά-hemolysin, 20 strains
of E. coli Hly +, 12 strains of C. albicans were detected using transmission
electron microscopy on a JEM-100C (JEOL, Japan).
Results:
With the manifestation of antagonistic activity of LB in relation to UPB
of various types, significant changes were found in all interacting cells.
Thus, extensive invaginations of intracytoplasmic membrane structures
appeared in LB cells, the formation of which indicated the activation of
metabolic processes in them.
Electron microscopic examination of co-grown cultures of LB with UPB and
C. albicans in places of close cell contact revealed significant destructive
changes in the cells of LB themselves. The main differences were in the
nature of the destruction of cell walls by the type of desquamation of small
layer-by-layer fragments of peptidoglycan layers. Along with destructive
changes in the cell wall, a specific change in the ultrafine structure of
the protein-ribosomal complex of the cytoplasm of lactobacilli was noted.
Conclusion:
Ultrastructural changes revealed during the joint cultivation of LB with S.
aureus, E. coli Hly +, and C. albicans testified to the strict specificity of the
interaction of these microorganisms.