TY - JOUR

T1 - Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization

AU - Tsvetov, Nikita

AU - Sereda, Lidia

AU - Korovkina, Anna

AU - Artemkina, Natalia

AU - Kozerozhets, Irina

AU - Samarov, Artemiy

N1 - Tsvetov, N., Sereda, L., Korovkina, A. et al. Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization. Biomass Conv. Bioref. 12 (Suppl 1), 145–156 (2022). https://doi.org/10.1007/s13399-022-02299-2 Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2022/7

Y1 - 2022/7

N2 - The present study has applied acid-based deep eutectic solvent containing choline chloride with malonic, malic, tartaric, and citric acids for ultrasound-assisted extraction of bioactive compounds from Empetrum hermafroditum Hager. Kinetics of the extraction process has been estimated in the framework of the second-order and film theory models obtaining time dependence of total phenolic compound yield and total flavonoid yield using Folin-Ciocalteu and AlCl3-complexation methods. Total antioxidant activity of extracts has been estimated using the phosphomolybdate method and DPPH radical assay. HPLC has determined that quercetin and kaempferol were present in the extracts. Temperature, time, and water addition to deep eutectic solvents (DESs) have been optimized for the extraction process using the Box-Behnken design. The optimal conditions for the extraction of polyphenolic compounds are 3 h at 45 °C with the addition of 50% water. Polyphenol yield achieves 320.8 mg mL−1. The optimal conditions for flavonoid extraction are 5 h at 60 °C with the addition of 30% water. Total flavonoid yield achieves 58.8 mg mL−1. Obtained data demonstrate DESs as promising extractants for bioactive component recovery from Empetrum hermafroditum Hager.

AB - The present study has applied acid-based deep eutectic solvent containing choline chloride with malonic, malic, tartaric, and citric acids for ultrasound-assisted extraction of bioactive compounds from Empetrum hermafroditum Hager. Kinetics of the extraction process has been estimated in the framework of the second-order and film theory models obtaining time dependence of total phenolic compound yield and total flavonoid yield using Folin-Ciocalteu and AlCl3-complexation methods. Total antioxidant activity of extracts has been estimated using the phosphomolybdate method and DPPH radical assay. HPLC has determined that quercetin and kaempferol were present in the extracts. Temperature, time, and water addition to deep eutectic solvents (DESs) have been optimized for the extraction process using the Box-Behnken design. The optimal conditions for the extraction of polyphenolic compounds are 3 h at 45 °C with the addition of 50% water. Polyphenol yield achieves 320.8 mg mL−1. The optimal conditions for flavonoid extraction are 5 h at 60 °C with the addition of 30% water. Total flavonoid yield achieves 58.8 mg mL−1. Obtained data demonstrate DESs as promising extractants for bioactive component recovery from Empetrum hermafroditum Hager.

KW - deep eutectic solvents

KW - Empetrum hermafroditum

KW - Ultrasound-assisted extraction

KW - kinetics

KW - Deep eutectic solvents

KW - Kinetics

UR - http://www.scopus.com/inward/record.url?scp=85122987565&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/548b4d7f-53e9-32da-9002-7b740f6dd6fe/

U2 - 10.1007/s13399-022-02299-2

DO - 10.1007/s13399-022-02299-2

M3 - Article

VL - 12

SP - 145

EP - 156

JO - Biomass Conversion and Biorefinery

JF - Biomass Conversion and Biorefinery

SN - 2190-6815

IS - Suppl 1

ER -