Research output: Contribution to journal › Article › peer-review
Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization. / Tsvetov, Nikita ; Sereda, Lidia ; Korovkina, Anna ; Artemkina, Natalia ; Kozerozhets, Irina ; Samarov, Artemiy .
In: Biomass Conversion and Biorefinery, Vol. 12, No. Suppl 1, 07.2022, p. 145-156.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization
AU - Tsvetov, Nikita
AU - Sereda, Lidia
AU - Korovkina, Anna
AU - Artemkina, Natalia
AU - Kozerozhets, Irina
AU - Samarov, Artemiy
N1 - Tsvetov, N., Sereda, L., Korovkina, A. et al. Ultrasound-assisted extraction of phytochemicals from Empetrum hermafroditum Hager. using acid-based deep eutectic solvent: kinetics and optimization. Biomass Conv. Bioref. 12 (Suppl 1), 145–156 (2022). https://doi.org/10.1007/s13399-022-02299-2 Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2022/7
Y1 - 2022/7
N2 - The present study has applied acid-based deep eutectic solvent containing choline chloride with malonic, malic, tartaric, and citric acids for ultrasound-assisted extraction of bioactive compounds from Empetrum hermafroditum Hager. Kinetics of the extraction process has been estimated in the framework of the second-order and film theory models obtaining time dependence of total phenolic compound yield and total flavonoid yield using Folin-Ciocalteu and AlCl3-complexation methods. Total antioxidant activity of extracts has been estimated using the phosphomolybdate method and DPPH radical assay. HPLC has determined that quercetin and kaempferol were present in the extracts. Temperature, time, and water addition to deep eutectic solvents (DESs) have been optimized for the extraction process using the Box-Behnken design. The optimal conditions for the extraction of polyphenolic compounds are 3 h at 45 °C with the addition of 50% water. Polyphenol yield achieves 320.8 mg mL−1. The optimal conditions for flavonoid extraction are 5 h at 60 °C with the addition of 30% water. Total flavonoid yield achieves 58.8 mg mL−1. Obtained data demonstrate DESs as promising extractants for bioactive component recovery from Empetrum hermafroditum Hager.
AB - The present study has applied acid-based deep eutectic solvent containing choline chloride with malonic, malic, tartaric, and citric acids for ultrasound-assisted extraction of bioactive compounds from Empetrum hermafroditum Hager. Kinetics of the extraction process has been estimated in the framework of the second-order and film theory models obtaining time dependence of total phenolic compound yield and total flavonoid yield using Folin-Ciocalteu and AlCl3-complexation methods. Total antioxidant activity of extracts has been estimated using the phosphomolybdate method and DPPH radical assay. HPLC has determined that quercetin and kaempferol were present in the extracts. Temperature, time, and water addition to deep eutectic solvents (DESs) have been optimized for the extraction process using the Box-Behnken design. The optimal conditions for the extraction of polyphenolic compounds are 3 h at 45 °C with the addition of 50% water. Polyphenol yield achieves 320.8 mg mL−1. The optimal conditions for flavonoid extraction are 5 h at 60 °C with the addition of 30% water. Total flavonoid yield achieves 58.8 mg mL−1. Obtained data demonstrate DESs as promising extractants for bioactive component recovery from Empetrum hermafroditum Hager.
KW - deep eutectic solvents
KW - Empetrum hermafroditum
KW - Ultrasound-assisted extraction
KW - kinetics
KW - Deep eutectic solvents
KW - Kinetics
UR - http://www.scopus.com/inward/record.url?scp=85122987565&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/548b4d7f-53e9-32da-9002-7b740f6dd6fe/
U2 - 10.1007/s13399-022-02299-2
DO - 10.1007/s13399-022-02299-2
M3 - Article
VL - 12
SP - 145
EP - 156
JO - Biomass Conversion and Biorefinery
JF - Biomass Conversion and Biorefinery
SN - 2190-6815
IS - Suppl 1
ER -
ID: 100212054