Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms. / Zhukov, Vladimir; Zorin, Evgeny; Zhernakov, Aleksandr; Afonin, Alexey; Akhtemova, Gulnar; Bovin, Andrej; Dolgikh, Aleksandra; Gorshkov, Artemii; Gribchenko, Emma; Ivanova, Kira; Kirienko, Anna; Kitaeva, Anna; Kliukova, Marina; Kulaeva, Olga; Kusakin, Pyotr; Leppyanen, Irina; Pavlova, Olga; Romanyuk, Daria; Rudaya, Elizaveta; Serova, Tatiana; Shtark, Oksana; Sulima, Anton; Tsyganova, Anna; Vasileva, Ekaterina; Dolgikh, Elena; Tsyganov, Viktor; Tikhonovich, Igor.
в: Biological Communications, Том 66, № 3, 12.11.2021, стр. 181-197.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms
AU - Zhukov, Vladimir
AU - Zorin, Evgeny
AU - Zhernakov, Aleksandr
AU - Afonin, Alexey
AU - Akhtemova, Gulnar
AU - Bovin, Andrej
AU - Dolgikh, Aleksandra
AU - Gorshkov, Artemii
AU - Gribchenko, Emma
AU - Ivanova, Kira
AU - Kirienko, Anna
AU - Kitaeva, Anna
AU - Kliukova, Marina
AU - Kulaeva, Olga
AU - Kusakin, Pyotr
AU - Leppyanen, Irina
AU - Pavlova, Olga
AU - Romanyuk, Daria
AU - Rudaya, Elizaveta
AU - Serova, Tatiana
AU - Shtark, Oksana
AU - Sulima, Anton
AU - Tsyganova, Anna
AU - Vasileva, Ekaterina
AU - Dolgikh, Elena
AU - Tsyganov, Viktor
AU - Tikhonovich, Igor
N1 - Publisher Copyright: Copyright: © 2021 Zhukov et al.
PY - 2021/11/12
Y1 - 2021/11/12
N2 - The garden pea (Pisum sativum L.), like most members of Fabaceae family, is capable of forming symbioses with beneficial soil microorganisms such as nodule bacteria (rhizobia), arbuscular mycorrhizal (AM) fungi and plant growth promoting bacteria (PGPB). The autoregulation of nodulation (AON) system is known to play an important role in controlling both the number of nodules and the level of root colonization by AM via root-to-shoot signaling mediated by CLAVATA/ ESR-related (CLE) peptides and their receptors. In the pea, mutations in genes Sym28 (CLV2-like) and Sym29 (CLV1-like), which encode receptors for CLE peptides, lead to the supernodulation phenotype, i.e., excessive nodule formation. The aim of the present study was to analyze the response of pea cv. ‘Frisson’ (wild type) and mutants P64 (sym28) and P88 (sym29) to complex inoculation with rhizobia, AM fungi and PGPB, with regard to biomass accumulation, yield and transcriptomic alterations. The plants were grown in quartz sand for 2 and 4 weeks after inoculation with either rhizobia (Rh) or complex inoculation with Rh + AM, Rh + PGPB, and Rh+AM+PGPB, and the biomass and yield were assessed. Transcriptome sequencing of whole shoots and roots was performed using a modified RNAseq protocol named MACE (Massive Analysis of cDNA Ends). In the experimental conditions, P88 (sym29) plants demonstrated the best biomass accumulation and yield, as compared to the wild type and P64 (sym28) plants, whereas P64 (sym28) had the lowest rate of biomass and seed yield. The transcriptome analysis showed that both supernodulating mutants more actively responded to biotic and abiotic factors than the wild-type plants and demonstrated increased expression of genes characteristic to late stages of nodule development. The roots of P64 (sym28) plants responded to AM+Rh treatment with upregulation of genes encoding plastid proteins, which can be connected with the activation of carotenoid biosynthesis (namely, the non-mevalonate pathway that takes place in root plastids). The more active response to symbionts in P88 (sym29) plants, as compared to cv. ‘Frisson’, was associated with counterregulation of transcripts involved in chloroplast functioning and development in leaves, which accompanies successful plant development in symbiotic conditions. Finally, the effect of retardation of plant aging upon mycorrhization on a transcriptomic level was recorded for cv. ‘Frisson’ but not for P64 (sym28) and P88 (sym29) mutants, which points towards its possible connection with the AON system. The results of this work link the plant’s autoregulation with the responsiveness to inoculation with beneficial soil microorganisms.
AB - The garden pea (Pisum sativum L.), like most members of Fabaceae family, is capable of forming symbioses with beneficial soil microorganisms such as nodule bacteria (rhizobia), arbuscular mycorrhizal (AM) fungi and plant growth promoting bacteria (PGPB). The autoregulation of nodulation (AON) system is known to play an important role in controlling both the number of nodules and the level of root colonization by AM via root-to-shoot signaling mediated by CLAVATA/ ESR-related (CLE) peptides and their receptors. In the pea, mutations in genes Sym28 (CLV2-like) and Sym29 (CLV1-like), which encode receptors for CLE peptides, lead to the supernodulation phenotype, i.e., excessive nodule formation. The aim of the present study was to analyze the response of pea cv. ‘Frisson’ (wild type) and mutants P64 (sym28) and P88 (sym29) to complex inoculation with rhizobia, AM fungi and PGPB, with regard to biomass accumulation, yield and transcriptomic alterations. The plants were grown in quartz sand for 2 and 4 weeks after inoculation with either rhizobia (Rh) or complex inoculation with Rh + AM, Rh + PGPB, and Rh+AM+PGPB, and the biomass and yield were assessed. Transcriptome sequencing of whole shoots and roots was performed using a modified RNAseq protocol named MACE (Massive Analysis of cDNA Ends). In the experimental conditions, P88 (sym29) plants demonstrated the best biomass accumulation and yield, as compared to the wild type and P64 (sym28) plants, whereas P64 (sym28) had the lowest rate of biomass and seed yield. The transcriptome analysis showed that both supernodulating mutants more actively responded to biotic and abiotic factors than the wild-type plants and demonstrated increased expression of genes characteristic to late stages of nodule development. The roots of P64 (sym28) plants responded to AM+Rh treatment with upregulation of genes encoding plastid proteins, which can be connected with the activation of carotenoid biosynthesis (namely, the non-mevalonate pathway that takes place in root plastids). The more active response to symbionts in P88 (sym29) plants, as compared to cv. ‘Frisson’, was associated with counterregulation of transcripts involved in chloroplast functioning and development in leaves, which accompanies successful plant development in symbiotic conditions. Finally, the effect of retardation of plant aging upon mycorrhization on a transcriptomic level was recorded for cv. ‘Frisson’ but not for P64 (sym28) and P88 (sym29) mutants, which points towards its possible connection with the AON system. The results of this work link the plant’s autoregulation with the responsiveness to inoculation with beneficial soil microorganisms.
KW - Arbuscular mycorrhiza
KW - Autoregulation of nodulation
KW - Complex inoculation
KW - Garden pea
KW - Nodule bacteria
KW - RNAseq
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85120417376&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/44fa5587-9fbf-3b69-b468-285ecf12957a/
U2 - 10.21638/spbu03.2021.301
DO - 10.21638/spbu03.2021.301
M3 - Article
AN - SCOPUS:85120417376
VL - 66
SP - 181
EP - 197
JO - Biological Communications
JF - Biological Communications
SN - 2542-2154
IS - 3
ER -
ID: 89724191