TY - JOUR

T1 - Synergy between Rhizobial Co-Microsymbionts Leads to an Increase in the Efficiency of Plant–Microbe Interactions

AU - Safronova, Vera

AU - Sazanova, Anna

AU - Belimov, Andrey

AU - Guro, Polina

AU - Kuznetsova, Irina

AU - Karlov, Denis

AU - Chirak, Elizaveta

AU - Yuzikhin, Oleg

AU - Verkhozina, Alla

AU - Afonin, Alexey

AU - Tikhonovich, Igor

PY - 2023/5/4

Y1 - 2023/5/4

N2 - Combined inoculation of legumes with rhizobia and plant growth-promoting rhizobacteria or endophytes is a known technique for increasing the efficiency of nitrogen-fixing symbiosis and plant productivity. The aim of this work was to expand knowledge about the synergistic effects between commercial rhizobia of pasture legumes and root nodule bacteria of relict legume species. Pot experiments were performed on common vetch ( Vicia sativa L.) and red clover ( Trifolium pratense L.) co-inoculated with the participation of the corresponding commercial rhizobial strains ( R. leguminosarum bv. viciae RCAM0626 and R. leguminosarum bv. trifolii RCAM1365) and seven strains isolated from nodules of relict legumes inhabiting the Baikal Lake region and the Altai Republic: Oxytropis popoviana, Astragalus chorinensis, O. tragacanthoides and Vicia costata. The inoculation of plants with combinations of strains (commercial strain plus the isolate from relict legume) had a different effect on symbiosis depending on the plant species: the increase in the number of nodules was mainly observed on vetch, whereas increased acetylene reduction activity was evident on clover. It was shown that the relict isolates differ significantly in the set of genes related to different genetic systems that affect plant-microbe interactions. At the same time, they had additional genes that are involved in the formation of symbiosis and determine its effectiveness, but are absent in the used commercial strains: symbiotic genes fix, nif, nod, noe and nol, as well as genes associated with the hormonal status of the plant and the processes of symbiogenesis ( acdRS, genes for gibberellins and auxins biosynthesis, genes of T3SS, T4SS and T6SS secretion systems). It can be expected that the accumulation of knowledge about microbial synergy on the example of the joint use of commercial and relict rhizobia will allow in the future the development of methods for the targeted selection of co-microsymbionts to increase the efficiency of agricultural legume-rhizobia systems.

AB - Combined inoculation of legumes with rhizobia and plant growth-promoting rhizobacteria or endophytes is a known technique for increasing the efficiency of nitrogen-fixing symbiosis and plant productivity. The aim of this work was to expand knowledge about the synergistic effects between commercial rhizobia of pasture legumes and root nodule bacteria of relict legume species. Pot experiments were performed on common vetch ( Vicia sativa L.) and red clover ( Trifolium pratense L.) co-inoculated with the participation of the corresponding commercial rhizobial strains ( R. leguminosarum bv. viciae RCAM0626 and R. leguminosarum bv. trifolii RCAM1365) and seven strains isolated from nodules of relict legumes inhabiting the Baikal Lake region and the Altai Republic: Oxytropis popoviana, Astragalus chorinensis, O. tragacanthoides and Vicia costata. The inoculation of plants with combinations of strains (commercial strain plus the isolate from relict legume) had a different effect on symbiosis depending on the plant species: the increase in the number of nodules was mainly observed on vetch, whereas increased acetylene reduction activity was evident on clover. It was shown that the relict isolates differ significantly in the set of genes related to different genetic systems that affect plant-microbe interactions. At the same time, they had additional genes that are involved in the formation of symbiosis and determine its effectiveness, but are absent in the used commercial strains: symbiotic genes fix, nif, nod, noe and nol, as well as genes associated with the hormonal status of the plant and the processes of symbiogenesis ( acdRS, genes for gibberellins and auxins biosynthesis, genes of T3SS, T4SS and T6SS secretion systems). It can be expected that the accumulation of knowledge about microbial synergy on the example of the joint use of commercial and relict rhizobia will allow in the future the development of methods for the targeted selection of co-microsymbionts to increase the efficiency of agricultural legume-rhizobia systems.

KW - crop legumes

KW - efficiency of symbiosis

KW - plant–microbe interaction

KW - relict symbiotic systems

KW - rhizobial synergy

UR - https://www.mendeley.com/catalogue/88182892-29d6-3471-936a-1d400c7fc027/

U2 - 10.3390/microorganisms11051206

DO - 10.3390/microorganisms11051206

M3 - Article

C2 - 37317180

VL - 11

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 5

M1 - 1206

ER -