TY - JOUR

T1 - Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity

AU - Titova, Maria V.

AU - Kochkin, Dmitry V.

AU - Sukhanova, Elena S.

AU - Gorshkova, Elena N.

AU - Tyurina, Tatiana M.

AU - Ivanov, Igor M.

AU - Lunkova, Maria K.

AU - Tsvetkova, Elena V.

AU - Orlova, Anastasia

AU - Popova, Elena V.

AU - Nosov, Alexander M.

PY - 2023/10/22

Y1 - 2023/10/22

N2 - Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complications, ischemic brain damage, and Parkinson's disease. Triterpene glycosides of the oleanane type, such as 3- O-[β- D-glucopyranosyl-(1→4)-β- D-glucuronopyranosyl] oleanolic acid 28- O-β- D-glucopyranosyl ester (PFS), ladyginoside A, and polysciosides A-H, are mainly responsible for biological activities of this species. In this study, cultivation of the cell suspension of P. fruticosa in 20 L bubble-type bioreactors was attempted as a sustainable method for cell biomass production of this valuable species and an alternative to overexploitation of wild plant resources. Cell suspension cultivated in bioreactors under a semi-continuous regime demonstrated satisfactory growth with a specific growth rate of 0.11 day -1, productivity of 0.32 g (L · day) -1, and an economic coefficient of 0.16 but slightly lower maximum biomass accumulation (~6.8 g L -1) compared to flask culture (~8.2 g L -1). Triterpene glycosides PFS (0.91 mg gDW -1) and ladyginoside A (0.77 mg gDW -1) were detected in bioreactor-produced cell biomass in higher concentrations compared to cells grown in flasks (0.50 and 0.22 mg gDW -1, respectively). In antibacterial tests, the minimum inhibitory concentrations (MICs) of cell biomass extracts against the most common pathogens Staphylococcus aureus, methicillin-resistant strain MRSA, Pseudomonas aeruginosa, and Escherichia coli varied within 250-2000 µg mL -1 which was higher compared to extracts of greenhouse plant leaves (MIC = 4000 µg mL -1). Cell biomass extracts also exhibited antioxidant activity, as confirmed by DPPH and TEAC assays. Our results suggest that bioreactor cultivation of P. fruticosa suspension cell culture may be a perspective method for the sustainable biomass production of this species.

AB - Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complications, ischemic brain damage, and Parkinson's disease. Triterpene glycosides of the oleanane type, such as 3- O-[β- D-glucopyranosyl-(1→4)-β- D-glucuronopyranosyl] oleanolic acid 28- O-β- D-glucopyranosyl ester (PFS), ladyginoside A, and polysciosides A-H, are mainly responsible for biological activities of this species. In this study, cultivation of the cell suspension of P. fruticosa in 20 L bubble-type bioreactors was attempted as a sustainable method for cell biomass production of this valuable species and an alternative to overexploitation of wild plant resources. Cell suspension cultivated in bioreactors under a semi-continuous regime demonstrated satisfactory growth with a specific growth rate of 0.11 day -1, productivity of 0.32 g (L · day) -1, and an economic coefficient of 0.16 but slightly lower maximum biomass accumulation (~6.8 g L -1) compared to flask culture (~8.2 g L -1). Triterpene glycosides PFS (0.91 mg gDW -1) and ladyginoside A (0.77 mg gDW -1) were detected in bioreactor-produced cell biomass in higher concentrations compared to cells grown in flasks (0.50 and 0.22 mg gDW -1, respectively). In antibacterial tests, the minimum inhibitory concentrations (MICs) of cell biomass extracts against the most common pathogens Staphylococcus aureus, methicillin-resistant strain MRSA, Pseudomonas aeruginosa, and Escherichia coli varied within 250-2000 µg mL -1 which was higher compared to extracts of greenhouse plant leaves (MIC = 4000 µg mL -1). Cell biomass extracts also exhibited antioxidant activity, as confirmed by DPPH and TEAC assays. Our results suggest that bioreactor cultivation of P. fruticosa suspension cell culture may be a perspective method for the sustainable biomass production of this species.

KW - Ming aralia

KW - PFS

KW - antimicrobial activity

KW - antioxidant activity

KW - cell aggregation

KW - cell farming

KW - ladyginoside A

KW - plant cell biotechnology

UR - https://www.mendeley.com/catalogue/9a38c79e-9349-3004-9266-95475c23e0ad/

U2 - 10.3390/plants12203641

DO - 10.3390/plants12203641

M3 - Article

C2 - 37896105

VL - 12

JO - Plants

JF - Plants

SN - 2223-7747

IS - 20

M1 - 3641

ER -