Research output: Contribution to journal › Article › peer-review
Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity. / Titova, Maria V.; Kochkin, Dmitry V.; Sukhanova, Elena S.; Gorshkova, Elena N.; Tyurina, Tatiana M.; Ivanov, Igor M.; Lunkova, Maria K.; Tsvetkova, Elena V. ; Orlova, Anastasia; Popova, Elena V.; Nosov, Alexander M.
In: Plants, Vol. 12, No. 20, 3641, 22.10.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Suspension Cell Culture of Polyscias fruticosa (L.) Harms in Bubble-Type Bioreactors—Growth Characteristics, Triterpene Glycosides Accumulation and Biological Activity
AU - Titova, Maria V.
AU - Kochkin, Dmitry V.
AU - Sukhanova, Elena S.
AU - Gorshkova, Elena N.
AU - Tyurina, Tatiana M.
AU - Ivanov, Igor M.
AU - Lunkova, Maria K.
AU - Tsvetkova, Elena V.
AU - Orlova, Anastasia
AU - Popova, Elena V.
AU - Nosov, Alexander M.
PY - 2023/10/22
Y1 - 2023/10/22
N2 - Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complications, ischemic brain damage, and Parkinson's disease. Triterpene glycosides of the oleanane type, such as 3- O-[β- D-glucopyranosyl-(1→4)-β- D-glucuronopyranosyl] oleanolic acid 28- O-β- D-glucopyranosyl ester (PFS), ladyginoside A, and polysciosides A-H, are mainly responsible for biological activities of this species. In this study, cultivation of the cell suspension of P. fruticosa in 20 L bubble-type bioreactors was attempted as a sustainable method for cell biomass production of this valuable species and an alternative to overexploitation of wild plant resources. Cell suspension cultivated in bioreactors under a semi-continuous regime demonstrated satisfactory growth with a specific growth rate of 0.11 day -1, productivity of 0.32 g (L · day) -1, and an economic coefficient of 0.16 but slightly lower maximum biomass accumulation (~6.8 g L -1) compared to flask culture (~8.2 g L -1). Triterpene glycosides PFS (0.91 mg gDW -1) and ladyginoside A (0.77 mg gDW -1) were detected in bioreactor-produced cell biomass in higher concentrations compared to cells grown in flasks (0.50 and 0.22 mg gDW -1, respectively). In antibacterial tests, the minimum inhibitory concentrations (MICs) of cell biomass extracts against the most common pathogens Staphylococcus aureus, methicillin-resistant strain MRSA, Pseudomonas aeruginosa, and Escherichia coli varied within 250-2000 µg mL -1 which was higher compared to extracts of greenhouse plant leaves (MIC = 4000 µg mL -1). Cell biomass extracts also exhibited antioxidant activity, as confirmed by DPPH and TEAC assays. Our results suggest that bioreactor cultivation of P. fruticosa suspension cell culture may be a perspective method for the sustainable biomass production of this species.
AB - Polyscias fruticosa (L.) Harms, or Ming aralia, is a medicinal plant of the Araliaceae family, which is highly valued for its antitoxic, anti-inflammatory, analgesic, antibacterial, anti-asthmatic, adaptogenic, and other properties. The plant can be potentially used to treat diabetes and its complications, ischemic brain damage, and Parkinson's disease. Triterpene glycosides of the oleanane type, such as 3- O-[β- D-glucopyranosyl-(1→4)-β- D-glucuronopyranosyl] oleanolic acid 28- O-β- D-glucopyranosyl ester (PFS), ladyginoside A, and polysciosides A-H, are mainly responsible for biological activities of this species. In this study, cultivation of the cell suspension of P. fruticosa in 20 L bubble-type bioreactors was attempted as a sustainable method for cell biomass production of this valuable species and an alternative to overexploitation of wild plant resources. Cell suspension cultivated in bioreactors under a semi-continuous regime demonstrated satisfactory growth with a specific growth rate of 0.11 day -1, productivity of 0.32 g (L · day) -1, and an economic coefficient of 0.16 but slightly lower maximum biomass accumulation (~6.8 g L -1) compared to flask culture (~8.2 g L -1). Triterpene glycosides PFS (0.91 mg gDW -1) and ladyginoside A (0.77 mg gDW -1) were detected in bioreactor-produced cell biomass in higher concentrations compared to cells grown in flasks (0.50 and 0.22 mg gDW -1, respectively). In antibacterial tests, the minimum inhibitory concentrations (MICs) of cell biomass extracts against the most common pathogens Staphylococcus aureus, methicillin-resistant strain MRSA, Pseudomonas aeruginosa, and Escherichia coli varied within 250-2000 µg mL -1 which was higher compared to extracts of greenhouse plant leaves (MIC = 4000 µg mL -1). Cell biomass extracts also exhibited antioxidant activity, as confirmed by DPPH and TEAC assays. Our results suggest that bioreactor cultivation of P. fruticosa suspension cell culture may be a perspective method for the sustainable biomass production of this species.
KW - Ming aralia
KW - PFS
KW - antimicrobial activity
KW - antioxidant activity
KW - cell aggregation
KW - cell farming
KW - ladyginoside A
KW - plant cell biotechnology
UR - https://www.mendeley.com/catalogue/9a38c79e-9349-3004-9266-95475c23e0ad/
U2 - 10.3390/plants12203641
DO - 10.3390/plants12203641
M3 - Article
C2 - 37896105
VL - 12
JO - Plants
JF - Plants
SN - 2223-7747
IS - 20
M1 - 3641
ER -
ID: 113609214