Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Simultaneous determination of rifabutin and human serum albumin in pharmaceutical formulations by capillary electrophoresis. / Ermolenko, Yu; Anshakova, A.; Osipova, N.; Kamentsev, M.; Maksimenko, O.; Balabanyan, V.; Gelperina, S.
в: Journal of Pharmacological and Toxicological Methods, Том 85, 01.05.2017, стр. 55-60.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Simultaneous determination of rifabutin and human serum albumin in pharmaceutical formulations by capillary electrophoresis
AU - Ermolenko, Yu
AU - Anshakova, A.
AU - Osipova, N.
AU - Kamentsev, M.
AU - Maksimenko, O.
AU - Balabanyan, V.
AU - Gelperina, S.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75 mm, effective length 50 cm, total length 60 cm), the capillary temperature was 25 °С, the voltage applied to the capillary tube was + 20 kV, the UV detection wavelength was 214 nm, hydrodynamic injection of the sample was performed at 30 mbar for 5 s, tetraborate buffer solution (0.01 М, рН 9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02 μg/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA).
AB - Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75 mm, effective length 50 cm, total length 60 cm), the capillary temperature was 25 °С, the voltage applied to the capillary tube was + 20 kV, the UV detection wavelength was 214 nm, hydrodynamic injection of the sample was performed at 30 mbar for 5 s, tetraborate buffer solution (0.01 М, рН 9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02 μg/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA).
KW - Capillary electrophoresis (CE)
KW - Human serum albumin (HSA)
KW - Rifabutin (RFB)
UR - http://www.scopus.com/inward/record.url?scp=85012239706&partnerID=8YFLogxK
U2 - 10.1016/j.vascn.2017.01.003
DO - 10.1016/j.vascn.2017.01.003
M3 - Article
AN - SCOPUS:85012239706
VL - 85
SP - 55
EP - 60
JO - Journal of Pharmacological and Toxicological Methods
JF - Journal of Pharmacological and Toxicological Methods
SN - 1056-8719
ER -
ID: 9517410