Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
SFP1-mediated prion-dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1. / Matveenko, Andrew G; Drozdova, Polina B; Belousov, Mikhail V; Moskalenko, Svetlana E; Bondarev, Stanislav A; Barbitoff, Yury A; Nizhnikov, Anton A; Zhouravleva, Galina A.
в: Genes to Cells, Том 21, № 12, 2016, стр. 1290-1308.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - SFP1-mediated prion-dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1
AU - Matveenko, Andrew G
AU - Drozdova, Polina B
AU - Belousov, Mikhail V
AU - Moskalenko, Svetlana E
AU - Bondarev, Stanislav A
AU - Barbitoff, Yury A
AU - Nizhnikov, Anton A
AU - Zhouravleva, Galina A
N1 - © 2016 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
PY - 2016
Y1 - 2016
N2 - [PSI+ ] is the prion form of the translation termination factor Sup35 (eRF3); [PSI+ ] strains display nonsense suppression. Another prion-like element, [ISP+ ], is linked to antisuppression in a specific background. Transcriptional regulator Sfp1 was shown to be responsible for [ISP+ ] propagation. In this work, we identified SFP1 as a multicopy inducer of [PSI+ ]-dependent lethality. Sfp1 is likely to up-regulate transcription of genes encoding release factors; however, its overproduction increases Sup35, but not Sup45 protein level. Using the synthetic lethality test, we compared the effects of SFP1 and SUP35 over-expression on the viability of [PSI+ ] strains. Together with an observation that Sfp1 overproduction leads to an increased accumulation of Sup35 in [PSI+ ] aggregates, we suggest that excess Sfp1 causes [PSI+ ] toxicity. Even though SUP45 over-expression is known to compensate for the [PSI+ ]-dependent lethality, it fails to do so when the lethality is caused by SFP1 over-expression. We discovered that the increased levels of Hsp40 chaperone Sis1 alleviate prion toxicity caused by either SFP1 or SUP35 over-expression and revert back to normal distribution of Sup35 between monomers and aggregate fractions. Finally, we showed that Sfp1 partially colocalizes with Sup35 aggregates, which may contribute to another mechanism of Sfp1-derived [PSI+ ] prion toxicity.
AB - [PSI+ ] is the prion form of the translation termination factor Sup35 (eRF3); [PSI+ ] strains display nonsense suppression. Another prion-like element, [ISP+ ], is linked to antisuppression in a specific background. Transcriptional regulator Sfp1 was shown to be responsible for [ISP+ ] propagation. In this work, we identified SFP1 as a multicopy inducer of [PSI+ ]-dependent lethality. Sfp1 is likely to up-regulate transcription of genes encoding release factors; however, its overproduction increases Sup35, but not Sup45 protein level. Using the synthetic lethality test, we compared the effects of SFP1 and SUP35 over-expression on the viability of [PSI+ ] strains. Together with an observation that Sfp1 overproduction leads to an increased accumulation of Sup35 in [PSI+ ] aggregates, we suggest that excess Sfp1 causes [PSI+ ] toxicity. Even though SUP45 over-expression is known to compensate for the [PSI+ ]-dependent lethality, it fails to do so when the lethality is caused by SFP1 over-expression. We discovered that the increased levels of Hsp40 chaperone Sis1 alleviate prion toxicity caused by either SFP1 or SUP35 over-expression and revert back to normal distribution of Sup35 between monomers and aggregate fractions. Finally, we showed that Sfp1 partially colocalizes with Sup35 aggregates, which may contribute to another mechanism of Sfp1-derived [PSI+ ] prion toxicity.
KW - DNA-Binding Proteins
KW - Genes, Fungal
KW - Genes, Lethal
KW - HSP40 Heat-Shock Proteins
KW - Mutation
KW - Peptide Termination Factors
KW - Prion Proteins
KW - Saccharomyces cerevisiae
KW - Saccharomyces cerevisiae Proteins
KW - Journal Article
U2 - 10.1111/gtc.12444
DO - 10.1111/gtc.12444
M3 - Article
C2 - 27734597
VL - 21
SP - 1290
EP - 1308
JO - Genes to Cells
JF - Genes to Cells
SN - 1356-9597
IS - 12
ER -
ID: 7594403