Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach. / Smolikova, Galina; Strygina, Ksenia; Krylova, Ekaterina; Vikhorev, Aleksander ; Билова, Татьяна Евгеньевна; Frolov, Andrej; Khlestkina, Elena; Medvedev, Sergei.
в: Plants, Том 11, № 13, 1686, 25.06.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach
AU - Smolikova, Galina
AU - Strygina, Ksenia
AU - Krylova, Ekaterina
AU - Vikhorev, Aleksander
AU - Билова, Татьяна Евгеньевна
AU - Frolov, Andrej
AU - Khlestkina, Elena
AU - Medvedev, Sergei
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6/25
Y1 - 2022/6/25
N2 - The seed-to-seedling transition is a crucial step in the plant life cycle. The transition occurs at the end of seed germination and corresponds to the initiation of embryonic root growth. To improve our understanding of how a seed transforms into a seedling, we germinated the Pisum sativum L. seeds for 72 h and divided them into samples before and after radicle protrusion. Before radicle protrusion, seeds survived after drying and formed normally developed seedlings upon rehydration. Radicle protrusion increased the moisture content level in seed axes, and the accumulation of ROS first generated in the embryonic root and plumule. The water and oxidative status shift correlated with the desiccation tolerance loss. Then, we compared RNA sequencing-based transcriptomics in the embryonic axes isolated from pea seeds before and after radicle protrusion. We identified 24,184 differentially expressed genes during the transition to the post-germination stage. Among them, 2101 genes showed more prominent expression. They were related to primary and secondary metabolism, photosynthesis, biosynthesis of cell wall components, redox status, and responses to biotic stress. On the other hand, 415 genes showed significantly decreased expression, including the groups related to water deprivation (eight genes) and response to the ABA stimulus (fifteen genes). We assume that the water deprivation group, especially three genes also belonging to ABA stimulus (LTI65, LTP4, and HVA22E), may be crucial for the desiccation tolerance loss during a metabolic switch from seed to seedling. The latter is also accompanied by the suppression of ABA-related transcription factors ABI3, ABI4, and ABI5. Among them, HVA22E, ABI4, and ABI5 were highly conservative in functional domains and showed homologous sequences in different drought-tolerant species. These findings elaborate on the critical biochemical pathways and genes regulating seed-to-seedling transition.
AB - The seed-to-seedling transition is a crucial step in the plant life cycle. The transition occurs at the end of seed germination and corresponds to the initiation of embryonic root growth. To improve our understanding of how a seed transforms into a seedling, we germinated the Pisum sativum L. seeds for 72 h and divided them into samples before and after radicle protrusion. Before radicle protrusion, seeds survived after drying and formed normally developed seedlings upon rehydration. Radicle protrusion increased the moisture content level in seed axes, and the accumulation of ROS first generated in the embryonic root and plumule. The water and oxidative status shift correlated with the desiccation tolerance loss. Then, we compared RNA sequencing-based transcriptomics in the embryonic axes isolated from pea seeds before and after radicle protrusion. We identified 24,184 differentially expressed genes during the transition to the post-germination stage. Among them, 2101 genes showed more prominent expression. They were related to primary and secondary metabolism, photosynthesis, biosynthesis of cell wall components, redox status, and responses to biotic stress. On the other hand, 415 genes showed significantly decreased expression, including the groups related to water deprivation (eight genes) and response to the ABA stimulus (fifteen genes). We assume that the water deprivation group, especially three genes also belonging to ABA stimulus (LTI65, LTP4, and HVA22E), may be crucial for the desiccation tolerance loss during a metabolic switch from seed to seedling. The latter is also accompanied by the suppression of ABA-related transcription factors ABI3, ABI4, and ABI5. Among them, HVA22E, ABI4, and ABI5 were highly conservative in functional domains and showed homologous sequences in different drought-tolerant species. These findings elaborate on the critical biochemical pathways and genes regulating seed-to-seedling transition.
KW - desiccation tolerance
KW - gene expression
KW - germination
KW - Pisum sativum L
KW - post-germination
KW - RNA-seq
KW - seeds
KW - transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85132708248&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/2fde85c8-32e1-3c36-b12f-d841e3250d3f/
U2 - 10.3390/plants11131686
DO - 10.3390/plants11131686
M3 - Article
C2 - 35807638
VL - 11
JO - Plants
JF - Plants
SN - 2223-7747
IS - 13
M1 - 1686
ER -
ID: 96614635