Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach

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Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach. / Smolikova, Galina ; Strygina, Ksenia ; Krylova, Ekaterina; Vikhorev, Aleksander ; Билова, Татьяна Евгеньевна; Frolov, Andrej; Khlestkina, Elena; Medvedev, Sergei.

In: Plants, Vol. 11, No. 13, 1686, 25.06.2022.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{7f8e22c8c0f24702905de7a2122c1d4a,

title = "Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach",

abstract = "The seed-to-seedling transition is a crucial step in the plant life cycle. The transition occurs at the end of seed germination and corresponds to the initiation of embryonic root growth. To improve our understanding of how a seed transforms into a seedling, we germinated the Pisum sativum L. seeds for 72 h and divided them into samples before and after radicle protrusion. Before radicle protrusion, seeds survived after drying and formed normally developed seedlings upon rehydration. Radicle protrusion increased the moisture content level in seed axes, and the accumulation of ROS first generated in the embryonic root and plumule. The water and oxidative status shift correlated with the desiccation tolerance loss. Then, we compared RNA sequencing-based transcriptomics in the embryonic axes isolated from pea seeds before and after radicle protrusion. We identified 24,184 differentially expressed genes during the transition to the post-germination stage. Among them, 2101 genes showed more prominent expression. They were related to primary and secondary metabolism, photosynthesis, biosynthesis of cell wall components, redox status, and responses to biotic stress. On the other hand, 415 genes showed significantly decreased expression, including the groups related to water deprivation (eight genes) and response to the ABA stimulus (fifteen genes). We assume that the water deprivation group, especially three genes also belonging to ABA stimulus (LTI65, LTP4, and HVA22E), may be crucial for the desiccation tolerance loss during a metabolic switch from seed to seedling. The latter is also accompanied by the suppression of ABA-related transcription factors ABI3, ABI4, and ABI5. Among them, HVA22E, ABI4, and ABI5 were highly conservative in functional domains and showed homologous sequences in different drought-tolerant species. These findings elaborate on the critical biochemical pathways and genes regulating seed-to-seedling transition.",

keywords = "desiccation tolerance, gene expression, germination, Pisum sativum L, post-germination, RNA-seq, seeds, transcriptome",

author = "Galina Smolikova and Ksenia Strygina and Ekaterina Krylova and Aleksander Vikhorev and Билова, {Татьяна Евгеньевна} and Andrej Frolov and Elena Khlestkina and Sergei Medvedev",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jun,

day = "25",

doi = "10.3390/plants11131686",

language = "English",

volume = "11",

journal = "Plants",

issn = "2223-7747",

publisher = "MDPI AG",

number = "13",

}

RIS

TY - JOUR

T1 - Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach

AU - Smolikova, Galina

AU - Strygina, Ksenia

AU - Krylova, Ekaterina

AU - Vikhorev, Aleksander

AU - Билова, Татьяна Евгеньевна

AU - Frolov, Andrej

AU - Khlestkina, Elena

AU - Medvedev, Sergei

PY - 2022/6/25

Y1 - 2022/6/25

N2 - The seed-to-seedling transition is a crucial step in the plant life cycle. The transition occurs at the end of seed germination and corresponds to the initiation of embryonic root growth. To improve our understanding of how a seed transforms into a seedling, we germinated the Pisum sativum L. seeds for 72 h and divided them into samples before and after radicle protrusion. Before radicle protrusion, seeds survived after drying and formed normally developed seedlings upon rehydration. Radicle protrusion increased the moisture content level in seed axes, and the accumulation of ROS first generated in the embryonic root and plumule. The water and oxidative status shift correlated with the desiccation tolerance loss. Then, we compared RNA sequencing-based transcriptomics in the embryonic axes isolated from pea seeds before and after radicle protrusion. We identified 24,184 differentially expressed genes during the transition to the post-germination stage. Among them, 2101 genes showed more prominent expression. They were related to primary and secondary metabolism, photosynthesis, biosynthesis of cell wall components, redox status, and responses to biotic stress. On the other hand, 415 genes showed significantly decreased expression, including the groups related to water deprivation (eight genes) and response to the ABA stimulus (fifteen genes). We assume that the water deprivation group, especially three genes also belonging to ABA stimulus (LTI65, LTP4, and HVA22E), may be crucial for the desiccation tolerance loss during a metabolic switch from seed to seedling. The latter is also accompanied by the suppression of ABA-related transcription factors ABI3, ABI4, and ABI5. Among them, HVA22E, ABI4, and ABI5 were highly conservative in functional domains and showed homologous sequences in different drought-tolerant species. These findings elaborate on the critical biochemical pathways and genes regulating seed-to-seedling transition.

AB - The seed-to-seedling transition is a crucial step in the plant life cycle. The transition occurs at the end of seed germination and corresponds to the initiation of embryonic root growth. To improve our understanding of how a seed transforms into a seedling, we germinated the Pisum sativum L. seeds for 72 h and divided them into samples before and after radicle protrusion. Before radicle protrusion, seeds survived after drying and formed normally developed seedlings upon rehydration. Radicle protrusion increased the moisture content level in seed axes, and the accumulation of ROS first generated in the embryonic root and plumule. The water and oxidative status shift correlated with the desiccation tolerance loss. Then, we compared RNA sequencing-based transcriptomics in the embryonic axes isolated from pea seeds before and after radicle protrusion. We identified 24,184 differentially expressed genes during the transition to the post-germination stage. Among them, 2101 genes showed more prominent expression. They were related to primary and secondary metabolism, photosynthesis, biosynthesis of cell wall components, redox status, and responses to biotic stress. On the other hand, 415 genes showed significantly decreased expression, including the groups related to water deprivation (eight genes) and response to the ABA stimulus (fifteen genes). We assume that the water deprivation group, especially three genes also belonging to ABA stimulus (LTI65, LTP4, and HVA22E), may be crucial for the desiccation tolerance loss during a metabolic switch from seed to seedling. The latter is also accompanied by the suppression of ABA-related transcription factors ABI3, ABI4, and ABI5. Among them, HVA22E, ABI4, and ABI5 were highly conservative in functional domains and showed homologous sequences in different drought-tolerant species. These findings elaborate on the critical biochemical pathways and genes regulating seed-to-seedling transition.

KW - desiccation tolerance

KW - gene expression

KW - germination

KW - Pisum sativum L

KW - post-germination

KW - RNA-seq

KW - seeds

KW - transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85132708248&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/2fde85c8-32e1-3c36-b12f-d841e3250d3f/

U2 - 10.3390/plants11131686

DO - 10.3390/plants11131686

M3 - Article

C2 - 35807638

VL - 11

JO - Plants

JF - Plants

SN - 2223-7747

IS - 13

M1 - 1686

ER -

ID: 96614635