Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Protocol for refining metagenomic binning with BinSPreader . / Очкалова, София Дмитриевна; Толстоганов, Иван Николаевич; Лапидус, Алла Львовна; Коробейников, Антон Иванович.
в: STAR Protocols, Том 4, № 3, 102417, 15.09.2023.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Protocol for refining metagenomic binning with BinSPreader
AU - Очкалова, София Дмитриевна
AU - Толстоганов, Иван Николаевич
AU - Лапидус, Алла Львовна
AU - Коробейников, Антон Иванович
PY - 2023/9/15
Y1 - 2023/9/15
N2 - The analysis of metagenomic data obtained via high-throughput DNA sequencing is primarily carried out by a dedicated binning process involving clustering contigs, presumably belonging to the same species. Here, we present a protocol for improving the quality of binning using BinSPreader. We describe steps for typical metagenome assembly and binning workflow. We then detail binning refining, its variants, output, and possible caveats. This protocol optimizes the process of reconstructing more complete genomes of microorganisms that make up the metagenome. For complete details on the use and execution of this protocol, please refer to Tolstoganov et al.1
AB - The analysis of metagenomic data obtained via high-throughput DNA sequencing is primarily carried out by a dedicated binning process involving clustering contigs, presumably belonging to the same species. Here, we present a protocol for improving the quality of binning using BinSPreader. We describe steps for typical metagenome assembly and binning workflow. We then detail binning refining, its variants, output, and possible caveats. This protocol optimizes the process of reconstructing more complete genomes of microorganisms that make up the metagenome. For complete details on the use and execution of this protocol, please refer to Tolstoganov et al.1
KW - Bioinformatics
KW - Genomics
KW - Sequence Analysis
KW - Sequencing
UR - https://www.mendeley.com/catalogue/4bd67041-f9e1-317b-a57e-a8e71e54cfbd/
U2 - 10.1016/j.xpro.2023.102417
DO - 10.1016/j.xpro.2023.102417
M3 - Article
VL - 4
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 3
M1 - 102417
ER -
ID: 106815185