TY - JOUR

T1 - Protocol for refining metagenomic binning with BinSPreader 

AU - Очкалова, София Дмитриевна

AU - Толстоганов, Иван Николаевич

AU - Лапидус, Алла Львовна

AU - Коробейников, Антон Иванович

PY - 2023/9/15

Y1 - 2023/9/15

N2 - The analysis of metagenomic data obtained via high-throughput DNA sequencing is primarily carried out by a dedicated binning process involving clustering contigs, presumably belonging to the same species. Here, we present a protocol for improving the quality of binning using BinSPreader. We describe steps for typical metagenome assembly and binning workflow. We then detail binning refining, its variants, output, and possible caveats. This protocol optimizes the process of reconstructing more complete genomes of microorganisms that make up the metagenome. For complete details on the use and execution of this protocol, please refer to Tolstoganov et al.1

AB - The analysis of metagenomic data obtained via high-throughput DNA sequencing is primarily carried out by a dedicated binning process involving clustering contigs, presumably belonging to the same species. Here, we present a protocol for improving the quality of binning using BinSPreader. We describe steps for typical metagenome assembly and binning workflow. We then detail binning refining, its variants, output, and possible caveats. This protocol optimizes the process of reconstructing more complete genomes of microorganisms that make up the metagenome. For complete details on the use and execution of this protocol, please refer to Tolstoganov et al.1

KW - Bioinformatics

KW - Genomics

KW - Sequence Analysis

KW - Sequencing

UR - https://www.mendeley.com/catalogue/4bd67041-f9e1-317b-a57e-a8e71e54cfbd/

U2 - 10.1016/j.xpro.2023.102417

DO - 10.1016/j.xpro.2023.102417

M3 - Article

VL - 4

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 3

M1 - 102417

ER -