Standard

Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton. / Vedyaykin, A. D.; Gorbunov, V. V.; Sabantsev, A. V.; Polinovskaya, V. S.; Vishnyakov, I. E.; Melnikov, A. S.; Serdobintsev, P. Yu; Khodorkovskii, M. A.

в: Journal of Physics: Conference Series, Том 643, № 1, 012020, 02.11.2015.

Результаты исследований: Научные публикации в периодических изданиях › статья в журнале по материалам конференции › Рецензирование

Harvard

Vedyaykin, AD, Gorbunov, VV, Sabantsev, AV, Polinovskaya, VS, Vishnyakov, IE, Melnikov, AS, Serdobintsev, PY & Khodorkovskii, MA 2015, 'Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton', Journal of Physics: Conference Series, Том. 643, № 1, 012020. https://doi.org/10.1088/1742-6596/643/1/012020

APA

Vedyaykin, A. D., Gorbunov, V. V., Sabantsev, A. V., Polinovskaya, V. S., Vishnyakov, I. E., Melnikov, A. S., Serdobintsev, P. Y., & Khodorkovskii, M. A. (2015). Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton. Journal of Physics: Conference Series, 643(1), [012020]. https://doi.org/10.1088/1742-6596/643/1/012020

Vancouver

Vedyaykin AD, Gorbunov VV, Sabantsev AV, Polinovskaya VS, Vishnyakov IE, Melnikov AS и пр. Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton. Journal of Physics: Conference Series. 2015 Нояб. 2;643(1). 012020. https://doi.org/10.1088/1742-6596/643/1/012020

Author

Vedyaykin, A. D. ; Gorbunov, V. V. ; Sabantsev, A. V. ; Polinovskaya, V. S. ; Vishnyakov, I. E. ; Melnikov, A. S. ; Serdobintsev, P. Yu ; Khodorkovskii, M. A. / Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton. в: Journal of Physics: Conference Series. 2015 ; Том 643, № 1.

BibTeX

@article{4a55098b9e984b7894ba2fe930e04dc3,
title = "Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton",
abstract = "Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. Localization microscopy was used to study FtsZ organization in Escherichia coli previously in combination with fluorescent protein labeling, but the fact that fluorescent chimeric protein was unable to rescue temperature-sensitive ftsZ mutants suggests that obtained images may not represent native FtsZ structures faithfully. Indirect immunolabeling of FtsZ not only overcomes this problem, but also allows the use of the powerful visualization methods arsenal available for different structures in fixed cells. In this work we simultaneously obtained super-resolution images of FtsZ structures and diffraction-limited or super-resolution images of DNA and cell surface in E. coli, which allows for the study of the spatial arrangement of FtsZ structures with respect to the nucleoid positions and septum formation.",
author = "Vedyaykin, {A. D.} and Gorbunov, {V. V.} and Sabantsev, {A. V.} and Polinovskaya, {V. S.} and Vishnyakov, {I. E.} and Melnikov, {A. S.} and Serdobintsev, {P. Yu} and Khodorkovskii, {M. A.}",
year = "2015",
month = nov,
day = "2",
doi = "10.1088/1742-6596/643/1/012020",
language = "English",
volume = "643",
journal = "Journal of Physics: Conference Series",
issn = "1742-6588",
publisher = "IOP Publishing Ltd.",
number = "1",
note = "2nd International School and Conference Saint-Petersburg OPEN on Optoelectronics, Photonics, Engineering and Nanostructures, SPbOPEN 2015 ; Conference date: 06-04-2015 Through 08-04-2015",

}

RIS

TY - JOUR

T1 - Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton

AU - Vedyaykin, A. D.

AU - Gorbunov, V. V.

AU - Sabantsev, A. V.

AU - Polinovskaya, V. S.

AU - Vishnyakov, I. E.

AU - Melnikov, A. S.

AU - Serdobintsev, P. Yu

AU - Khodorkovskii, M. A.

PY - 2015/11/2

Y1 - 2015/11/2

N2 - Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. Localization microscopy was used to study FtsZ organization in Escherichia coli previously in combination with fluorescent protein labeling, but the fact that fluorescent chimeric protein was unable to rescue temperature-sensitive ftsZ mutants suggests that obtained images may not represent native FtsZ structures faithfully. Indirect immunolabeling of FtsZ not only overcomes this problem, but also allows the use of the powerful visualization methods arsenal available for different structures in fixed cells. In this work we simultaneously obtained super-resolution images of FtsZ structures and diffraction-limited or super-resolution images of DNA and cell surface in E. coli, which allows for the study of the spatial arrangement of FtsZ structures with respect to the nucleoid positions and septum formation.

AB - Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. Localization microscopy was used to study FtsZ organization in Escherichia coli previously in combination with fluorescent protein labeling, but the fact that fluorescent chimeric protein was unable to rescue temperature-sensitive ftsZ mutants suggests that obtained images may not represent native FtsZ structures faithfully. Indirect immunolabeling of FtsZ not only overcomes this problem, but also allows the use of the powerful visualization methods arsenal available for different structures in fixed cells. In this work we simultaneously obtained super-resolution images of FtsZ structures and diffraction-limited or super-resolution images of DNA and cell surface in E. coli, which allows for the study of the spatial arrangement of FtsZ structures with respect to the nucleoid positions and septum formation.

UR - http://www.scopus.com/inward/record.url?scp=84952943894&partnerID=8YFLogxK

U2 - 10.1088/1742-6596/643/1/012020

DO - 10.1088/1742-6596/643/1/012020

M3 - Conference article

AN - SCOPUS:84952943894

VL - 643

JO - Journal of Physics: Conference Series

JF - Journal of Physics: Conference Series

SN - 1742-6588

IS - 1

M1 - 012020

T2 - 2nd International School and Conference Saint-Petersburg OPEN on Optoelectronics, Photonics, Engineering and Nanostructures, SPbOPEN 2015

Y2 - 6 April 2015 through 8 April 2015

ER -

ID: 35360614