Research output: Contribution to journal › Conference article › peer-review
Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton. / Vedyaykin, A. D.; Gorbunov, V. V.; Sabantsev, A. V.; Polinovskaya, V. S.; Vishnyakov, I. E.; Melnikov, A. S.; Serdobintsev, P. Yu; Khodorkovskii, M. A.
In: Journal of Physics: Conference Series, Vol. 643, No. 1, 012020, 02.11.2015.Research output: Contribution to journal › Conference article › peer-review
}
TY - JOUR
T1 - Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton
AU - Vedyaykin, A. D.
AU - Gorbunov, V. V.
AU - Sabantsev, A. V.
AU - Polinovskaya, V. S.
AU - Vishnyakov, I. E.
AU - Melnikov, A. S.
AU - Serdobintsev, P. Yu
AU - Khodorkovskii, M. A.
PY - 2015/11/2
Y1 - 2015/11/2
N2 - Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. Localization microscopy was used to study FtsZ organization in Escherichia coli previously in combination with fluorescent protein labeling, but the fact that fluorescent chimeric protein was unable to rescue temperature-sensitive ftsZ mutants suggests that obtained images may not represent native FtsZ structures faithfully. Indirect immunolabeling of FtsZ not only overcomes this problem, but also allows the use of the powerful visualization methods arsenal available for different structures in fixed cells. In this work we simultaneously obtained super-resolution images of FtsZ structures and diffraction-limited or super-resolution images of DNA and cell surface in E. coli, which allows for the study of the spatial arrangement of FtsZ structures with respect to the nucleoid positions and septum formation.
AB - Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. Localization microscopy was used to study FtsZ organization in Escherichia coli previously in combination with fluorescent protein labeling, but the fact that fluorescent chimeric protein was unable to rescue temperature-sensitive ftsZ mutants suggests that obtained images may not represent native FtsZ structures faithfully. Indirect immunolabeling of FtsZ not only overcomes this problem, but also allows the use of the powerful visualization methods arsenal available for different structures in fixed cells. In this work we simultaneously obtained super-resolution images of FtsZ structures and diffraction-limited or super-resolution images of DNA and cell surface in E. coli, which allows for the study of the spatial arrangement of FtsZ structures with respect to the nucleoid positions and septum formation.
UR - http://www.scopus.com/inward/record.url?scp=84952943894&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/643/1/012020
DO - 10.1088/1742-6596/643/1/012020
M3 - Conference article
AN - SCOPUS:84952943894
VL - 643
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
SN - 1742-6588
IS - 1
M1 - 012020
T2 - 2nd International School and Conference Saint-Petersburg OPEN on Optoelectronics, Photonics, Engineering and Nanostructures, SPbOPEN 2015
Y2 - 6 April 2015 through 8 April 2015
ER -
ID: 35360614