TY - JOUR

T1 - Microextraction of sulfonamides from chicken meat samples in three-component deep eutectic solvent

AU - Shishov, Andrey

AU - Gorbunov, Artem

AU - Baranovskii, Egor

AU - Bulatov, Andrey

N1 - Funding Information: The study was supported by a grant from the President of the Russian Federation MK-1154.2020.3. Scientific research was partially performed at Chemical Analysis and Materials Research Centre of Saint Petersburg State University . Publisher Copyright: © 2020 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11

Y1 - 2020/11

N2 - An effective approach for the sample pretreatment of solid food samples based on the use of three-component deep eutectic solvent (quaternary ammonium salt, carboxylic acid and medium chain fatty acid) was developed. Each component of deep eutectic solvent played a key role for separation of target analytes from solid-phase sample and their preconcentration based on dispersive liquid–liquid microextraction. The approach was applied for the HPLC-UV determination of sulfamethazine and sulfamethoxazole in chicken meat samples. The effect of deep eutectic solvents composition on the efficiency of both sample pretreatment steps was investigated. It was found that the solvent based on tetrabutylammonium bromide, malonic acid and hexanoic acid (1:1:1, mol/mol) provided the most effective separation and preconcentration of sulfanilamides. The procedure included mixing of solid food sample and deep eutectic solvent. By this sample pretreatment step, sulfanilamides were extracted into the solvent phase. For preconcentration of sulfanilamides, aqueous phase was injected into the deep eutectic solvent phase, resulting in its decomposition and the formation of hexanoic acid dispersed in aqueous phase. After phase separation, hexanoic acid phase containing sulfanilamides was withdrawn for HPLC-UV analysis. Under optimal conditions, the limits of detection calculated from a blank test based on 3σ were 3 µg kg−1 and 7 µg kg−1 for sulfamethoxazole and sulfamethazine, respectively.

AB - An effective approach for the sample pretreatment of solid food samples based on the use of three-component deep eutectic solvent (quaternary ammonium salt, carboxylic acid and medium chain fatty acid) was developed. Each component of deep eutectic solvent played a key role for separation of target analytes from solid-phase sample and their preconcentration based on dispersive liquid–liquid microextraction. The approach was applied for the HPLC-UV determination of sulfamethazine and sulfamethoxazole in chicken meat samples. The effect of deep eutectic solvents composition on the efficiency of both sample pretreatment steps was investigated. It was found that the solvent based on tetrabutylammonium bromide, malonic acid and hexanoic acid (1:1:1, mol/mol) provided the most effective separation and preconcentration of sulfanilamides. The procedure included mixing of solid food sample and deep eutectic solvent. By this sample pretreatment step, sulfanilamides were extracted into the solvent phase. For preconcentration of sulfanilamides, aqueous phase was injected into the deep eutectic solvent phase, resulting in its decomposition and the formation of hexanoic acid dispersed in aqueous phase. After phase separation, hexanoic acid phase containing sulfanilamides was withdrawn for HPLC-UV analysis. Under optimal conditions, the limits of detection calculated from a blank test based on 3σ were 3 µg kg−1 and 7 µg kg−1 for sulfamethoxazole and sulfamethazine, respectively.

KW - Deep eutectic solvent

KW - Dispersive liquid–liquid microextraction

KW - Food sample

KW - HPLC-UV

KW - Sample pretreatment

KW - Sulfonamides

KW - PRECONCENTRATION

KW - SOLIDIFICATION

KW - FRUIT JUICE

KW - CHROMATOGRAPHY

KW - EXTRACTION

KW - TRACE ANALYSIS

KW - LIQUID-LIQUID MICROEXTRACTION

KW - Dispersive liquid-liquid microextraction

KW - PESTICIDE-RESIDUES

KW - EFFICIENT

KW - SPECTROMETRY

UR - http://www.scopus.com/inward/record.url?scp=85088015006&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/8cde386e-b11b-3143-a688-50e363a65090/

U2 - 10.1016/j.microc.2020.105274

DO - 10.1016/j.microc.2020.105274

M3 - Article

AN - SCOPUS:85088015006

VL - 158

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

M1 - 105274

ER -