TY - JOUR

T1 - MICRO-CT AS A METHOD TO VISUALIZE INTRAMOLLUSCAN STAGES OF DIGENEA

AU - Kremnev, G.A.

AU - Kryuchkova, L.Y.

AU - Miroliubov, A.A.

AU - Kalashnikova, V.A.

AU - Krupenko, D.Y.

PY - 2020/2/22

Y1 - 2020/2/22

N2 - X-ray micro-computed tomography (micro-CT) is a non-destructive method widely used for visualization of three-dimensional structures. Application of micro-CT for comparative morphology is limited due to low x-ray contrast of soft animal tissues, but staining can increase image quality for such specimens. We suggest that micro-CT may be used for rough visualization of branched sporocysts of Digenea within intact hosts, and tested this approach on sporocyst of Leucochloridium paradoxum . Two infected mollusks were treated following two different protocols. One specimen was scanned in ethanol; the other was dried before scanning. Anatomical features of the host were better visible on microtomographic sections of the dried specimen. Regardless of the sample preparation, full-grown and underdeveloped broodsacs of the sporocyst were visible, but we could not trace its central part. We suggest how the micro-CT protocol can be modified for better results on branched digenean sporocysts.

AB - X-ray micro-computed tomography (micro-CT) is a non-destructive method widely used for visualization of three-dimensional structures. Application of micro-CT for comparative morphology is limited due to low x-ray contrast of soft animal tissues, but staining can increase image quality for such specimens. We suggest that micro-CT may be used for rough visualization of branched sporocysts of Digenea within intact hosts, and tested this approach on sporocyst of Leucochloridium paradoxum . Two infected mollusks were treated following two different protocols. One specimen was scanned in ethanol; the other was dried before scanning. Anatomical features of the host were better visible on microtomographic sections of the dried specimen. Regardless of the sample preparation, full-grown and underdeveloped broodsacs of the sporocyst were visible, but we could not trace its central part. We suggest how the micro-CT protocol can be modified for better results on branched digenean sporocysts.

KW - КОМПЬЮТЕРНАЯ МИКРОТОМОГРАФИЯ, ФОСФОРНОВОЛЬФРАМОВАЯ КИСЛОТА, СУШКА ЧЕРЕЗ КРИТИЧЕСКУЮ ТОЧКУ, ТРЕМАТОДЫ, LEUCOCHLORIDIUM PARADOXUM, СПОРОЦИСТА, МЕТАЦЕРКАРИИ, GASTROPODA, SUCCINEA PUTRIS, MICRO-CT, PHOSPHOTUNGSTIC ACID, CRITICAL POINT DRYING, DIGENEA, LEUCOCH

KW - LEUCOCHLORIDIUM PARADOXUM

KW - Gastropoda

KW - SUCCINEA PUTRIS

KW - Micro-CT

KW - Phosphotungstic acid

KW - CRITICAL POINT DRYING

KW - Digenea

KW - LEUCOCHLORIDIUM PAR ADOXUM

KW - Sporocysts

KW - metacercariae

UR - https://www.mendeley.com/catalogue/b11883ba-ed23-39e3-b88b-153758a39081/

U2 - 10.31857/S1234567806040045

DO - 10.31857/S1234567806040045

M3 - Article

VL - 54

SP - 312

EP - 321

JO - ПАРАЗИТОЛОГИЯ

JF - ПАРАЗИТОЛОГИЯ

SN - 0031-1847

IS - 4

ER -