MICRO-CT AS A METHOD TO VISUALIZE INTRAMOLLUSCAN STAGES OF DIGENEA

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MICRO-CT AS A METHOD TO VISUALIZE INTRAMOLLUSCAN STAGES OF DIGENEA. / Kremnev, G.A.; Kryuchkova, L.Y.; Miroliubov, A.A.; Kalashnikova, V.A.; Krupenko, D.Y.

In: Parazitologiya, Vol. 54, No. 4, 22.02.2020, p. 312-321.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{1655ea33e5744dce882f30e6ddab5ac1,

title = "MICRO-CT AS A METHOD TO VISUALIZE INTRAMOLLUSCAN STAGES OF DIGENEA",

abstract = "X-ray micro-computed tomography (micro-CT) is a non-destructive method widely used for visualization of three-dimensional structures. Application of micro-CT for comparative morphology is limited due to low x-ray contrast of soft animal tissues, but staining can increase image quality for such specimens. We suggest that micro-CT may be used for rough visualization of branched sporocysts of Digenea within intact hosts, and tested this approach on sporocyst of Leucochloridium paradoxum . Two infected mollusks were treated following two different protocols. One specimen was scanned in ethanol; the other was dried before scanning. Anatomical features of the host were better visible on microtomographic sections of the dried specimen. Regardless of the sample preparation, full-grown and underdeveloped broodsacs of the sporocyst were visible, but we could not trace its central part. We suggest how the micro-CT protocol can be modified for better results on branched digenean sporocysts.",

keywords = "КОМПЬЮТЕРНАЯ МИКРОТОМОГРАФИЯ, ФОСФОРНОВОЛЬФРАМОВАЯ КИСЛОТА, СУШКА ЧЕРЕЗ КРИТИЧЕСКУЮ ТОЧКУ, ТРЕМАТОДЫ, LEUCOCHLORIDIUM PARADOXUM, СПОРОЦИСТА, МЕТАЦЕРКАРИИ, GASTROPODA, SUCCINEA PUTRIS, MICRO-CT, PHOSPHOTUNGSTIC ACID, CRITICAL POINT DRYING, DIGENEA, LEUCOCHLORIDIUM PAR ADOXUM, SPOROCYST, METACERCARIAE, LEUCOCHLORIDIUM PARADOXUM, Gastropoda, SUCCINEA PUTRIS, Micro-CT, Phosphotungstic acid, CRITICAL POINT DRYING, Digenea, LEUCOCHLORIDIUM PAR ADOXUM, Sporocysts, metacercariae",

author = "G.A. Kremnev and L.Y. Kryuchkova and A.A. Miroliubov and V.A. Kalashnikova and D.Y. Krupenko",

year = "2020",

month = feb,

day = "22",

doi = "10.31857/S1234567806040045",

language = "English",

volume = "54",

pages = "312--321",

journal = "ПАРАЗИТОЛОГИЯ",

issn = "0031-1847",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "4",

}

RIS

TY - JOUR

T1 - MICRO-CT AS A METHOD TO VISUALIZE INTRAMOLLUSCAN STAGES OF DIGENEA

AU - Kremnev, G.A.

AU - Kryuchkova, L.Y.

AU - Miroliubov, A.A.

AU - Kalashnikova, V.A.

AU - Krupenko, D.Y.

PY - 2020/2/22

Y1 - 2020/2/22

N2 - X-ray micro-computed tomography (micro-CT) is a non-destructive method widely used for visualization of three-dimensional structures. Application of micro-CT for comparative morphology is limited due to low x-ray contrast of soft animal tissues, but staining can increase image quality for such specimens. We suggest that micro-CT may be used for rough visualization of branched sporocysts of Digenea within intact hosts, and tested this approach on sporocyst of Leucochloridium paradoxum . Two infected mollusks were treated following two different protocols. One specimen was scanned in ethanol; the other was dried before scanning. Anatomical features of the host were better visible on microtomographic sections of the dried specimen. Regardless of the sample preparation, full-grown and underdeveloped broodsacs of the sporocyst were visible, but we could not trace its central part. We suggest how the micro-CT protocol can be modified for better results on branched digenean sporocysts.

AB - X-ray micro-computed tomography (micro-CT) is a non-destructive method widely used for visualization of three-dimensional structures. Application of micro-CT for comparative morphology is limited due to low x-ray contrast of soft animal tissues, but staining can increase image quality for such specimens. We suggest that micro-CT may be used for rough visualization of branched sporocysts of Digenea within intact hosts, and tested this approach on sporocyst of Leucochloridium paradoxum . Two infected mollusks were treated following two different protocols. One specimen was scanned in ethanol; the other was dried before scanning. Anatomical features of the host were better visible on microtomographic sections of the dried specimen. Regardless of the sample preparation, full-grown and underdeveloped broodsacs of the sporocyst were visible, but we could not trace its central part. We suggest how the micro-CT protocol can be modified for better results on branched digenean sporocysts.

KW - КОМПЬЮТЕРНАЯ МИКРОТОМОГРАФИЯ, ФОСФОРНОВОЛЬФРАМОВАЯ КИСЛОТА, СУШКА ЧЕРЕЗ КРИТИЧЕСКУЮ ТОЧКУ, ТРЕМАТОДЫ, LEUCOCHLORIDIUM PARADOXUM, СПОРОЦИСТА, МЕТАЦЕРКАРИИ, GASTROPODA, SUCCINEA PUTRIS, MICRO-CT, PHOSPHOTUNGSTIC ACID, CRITICAL POINT DRYING, DIGENEA, LEUCOCH

KW - LEUCOCHLORIDIUM PARADOXUM

KW - Gastropoda

KW - SUCCINEA PUTRIS

KW - Micro-CT

KW - Phosphotungstic acid

KW - CRITICAL POINT DRYING

KW - Digenea

KW - LEUCOCHLORIDIUM PAR ADOXUM

KW - Sporocysts

KW - metacercariae

UR - https://www.mendeley.com/catalogue/b11883ba-ed23-39e3-b88b-153758a39081/

U2 - 10.31857/S1234567806040045

DO - 10.31857/S1234567806040045

M3 - Article

VL - 54

SP - 312

EP - 321

JO - ПАРАЗИТОЛОГИЯ

JF - ПАРАЗИТОЛОГИЯ

SN - 0031-1847

IS - 4

ER -

ID: 69965212