Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation. / Лобов, Арсений Андреевич; Кучур , Полина; Хижина, Айгуль Альфретовна; Котова, Анастасия Викторовна; Ивашкин, Андрей ; Костина, Дарья ; Клаузен, Полина; Хохлова, Евгения; Репкин, Егор Алексеевич; Постникова, Ксения ; Переплетчикова , Дарья ; Денисов, Евгений ; Геращенко , Татьяна ; Тихилов, Рашид; Божкова, Светлана; Середа , Андрей ; Карелкин, Виталий ; Енукашвили , Нателла; Малашичева, Анна Борисовна.
в: Stem Cells, 01.11.2023.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation
AU - Лобов, Арсений Андреевич
AU - Кучур , Полина
AU - Хижина, Айгуль Альфретовна
AU - Котова, Анастасия Викторовна
AU - Ивашкин, Андрей
AU - Костина, Дарья
AU - Клаузен, Полина
AU - Хохлова, Евгения
AU - Репкин, Егор Алексеевич
AU - Постникова, Ксения
AU - Переплетчикова , Дарья
AU - Денисов, Евгений
AU - Геращенко , Татьяна
AU - Тихилов, Рашид
AU - Божкова, Светлана
AU - Середа , Андрей
AU - Карелкин, Виталий
AU - Енукашвили , Нателла
AU - Малашичева, Анна Борисовна
PY - 2023/11/1
Y1 - 2023/11/1
N2 - Mesenchymal stem cells (MSCs) are widely used in therapy, but the differences between MSCs of various origins and their ability to undergo osteogenic differentiation and produce extracellular matrix are not fully understood. To address this, we conducted a comparative analysis of mesenchymal cell primary cultures from six human sources, including osteoblast-like cells from the adult femur, adipose-derived stem cells, Wharton's jelly-derived mesenchymal cells, gingival fibroblasts, dental pulp stem cells, and periodontal ligament stem cells. We analyzed these cells' secretome, proteome, and transcriptome under standard and osteogenic cultivation conditions. Despite the overall similarity in osteogenic differentiation, the cells maintain their embryonic specificity after isolation and differentiation in vitro. Furthermore, we propose classifying mesenchymal cells into three groups: dental stem cells of neural crest origin, mesenchymal stem cells, and fetal stem cells. Specifically, fetal stem cells have the most promising secretome for various applications, while mesenchymal stem cells have a specialized secretome optimal for extracellular matrix production. Nevertheless, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. In conclusion, our study illuminates the distinctive characteristics of mesenchymal stem cells from various sources, providing insights into their potential applications in regenerative medicine and enhancing our understanding of the inherent diversity of mesenchymal cells in vivo.
AB - Mesenchymal stem cells (MSCs) are widely used in therapy, but the differences between MSCs of various origins and their ability to undergo osteogenic differentiation and produce extracellular matrix are not fully understood. To address this, we conducted a comparative analysis of mesenchymal cell primary cultures from six human sources, including osteoblast-like cells from the adult femur, adipose-derived stem cells, Wharton's jelly-derived mesenchymal cells, gingival fibroblasts, dental pulp stem cells, and periodontal ligament stem cells. We analyzed these cells' secretome, proteome, and transcriptome under standard and osteogenic cultivation conditions. Despite the overall similarity in osteogenic differentiation, the cells maintain their embryonic specificity after isolation and differentiation in vitro. Furthermore, we propose classifying mesenchymal cells into three groups: dental stem cells of neural crest origin, mesenchymal stem cells, and fetal stem cells. Specifically, fetal stem cells have the most promising secretome for various applications, while mesenchymal stem cells have a specialized secretome optimal for extracellular matrix production. Nevertheless, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. In conclusion, our study illuminates the distinctive characteristics of mesenchymal stem cells from various sources, providing insights into their potential applications in regenerative medicine and enhancing our understanding of the inherent diversity of mesenchymal cells in vivo.
KW - allosteric modulators
KW - brain disorders
KW - gpcr
KW - gpcr biased signaling
KW - heterodimers
KW - mglu receptor
UR - https://academic.oup.com/stmcls/advance-article/doi/10.1093/stmcls/sxad081/7336816
UR - https://www.mendeley.com/catalogue/49adcdc8-1dfb-33dd-bdc6-bdf1b972f813/
U2 - 10.1093/stmcls/sxad081
DO - 10.1093/stmcls/sxad081
M3 - Article
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
M1 - sxad081
ER -
ID: 113871097