Standard

Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation. / Лобов, Арсений Андреевич; Кучур , Полина; Хижина, Айгуль Альфретовна; Котова, Анастасия Викторовна; Ивашкин, Андрей ; Костина, Дарья ; Клаузен, Полина; Хохлова, Евгения; Репкин, Егор Алексеевич; Постникова, Ксения ; Переплетчикова , Дарья ; Денисов, Евгений ; Геращенко , Татьяна ; Тихилов, Рашид; Божкова, Светлана; Середа , Андрей ; Карелкин, Виталий ; Енукашвили , Нателла; Малашичева, Анна Борисовна.

In: Stem Cells, 01.11.2023.

Research output: Contribution to journal › Article › peer-review

Harvard

Лобов, АА, Кучур , П, Хижина, АА, Котова, АВ, Ивашкин, А, Костина, Д, Клаузен, П, Хохлова, Е, Репкин, ЕА, Постникова, К, Переплетчикова , Д, Денисов, Е, Геращенко , Т, Тихилов, Р, Божкова, С, Середа , А, Карелкин, В, Енукашвили , Н & Малашичева, АБ 2023, 'Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation', Stem Cells. https://doi.org/10.1093/stmcls/sxad081

APA

Лобов, А. А., Кучур , П., Хижина, А. А., Котова, А. В., Ивашкин, А., Костина, Д., Клаузен, П., Хохлова, Е., Репкин, Е. А., Постникова, К., Переплетчикова , Д., Денисов, Е., Геращенко , Т., Тихилов, Р., Божкова, С., Середа , А., Карелкин, В., Енукашвили , Н., & Малашичева, А. Б. (2023). Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation. Stem Cells, [sxad081]. https://doi.org/10.1093/stmcls/sxad081

Vancouver

Лобов АА, Кучур П, Хижина АА, Котова АВ, Ивашкин А, Костина Д et al. Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation. Stem Cells. 2023 Nov 1. sxad081. https://doi.org/10.1093/stmcls/sxad081

Author

Лобов, Арсений Андреевич ; Кучур , Полина ; Хижина, Айгуль Альфретовна ; Котова, Анастасия Викторовна ; Ивашкин, Андрей ; Костина, Дарья ; Клаузен, Полина ; Хохлова, Евгения ; Репкин, Егор Алексеевич ; Постникова, Ксения ; Переплетчикова , Дарья ; Денисов, Евгений ; Геращенко , Татьяна ; Тихилов, Рашид ; Божкова, Светлана ; Середа , Андрей ; Карелкин, Виталий ; Енукашвили , Нателла ; Малашичева, Анна Борисовна. / Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation. In: Stem Cells. 2023.

BibTeX

@article{818f32939bd34b28b7fae0c791436c28,
title = "Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation",
abstract = "Mesenchymal stem cells (MSCs) are widely used in therapy, but the differences between MSCs of various origins and their ability to undergo osteogenic differentiation and produce extracellular matrix are not fully understood. To address this, we conducted a comparative analysis of mesenchymal cell primary cultures from six human sources, including osteoblast-like cells from the adult femur, adipose-derived stem cells, Wharton's jelly-derived mesenchymal cells, gingival fibroblasts, dental pulp stem cells, and periodontal ligament stem cells. We analyzed these cells' secretome, proteome, and transcriptome under standard and osteogenic cultivation conditions. Despite the overall similarity in osteogenic differentiation, the cells maintain their embryonic specificity after isolation and differentiation in vitro. Furthermore, we propose classifying mesenchymal cells into three groups: dental stem cells of neural crest origin, mesenchymal stem cells, and fetal stem cells. Specifically, fetal stem cells have the most promising secretome for various applications, while mesenchymal stem cells have a specialized secretome optimal for extracellular matrix production. Nevertheless, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. In conclusion, our study illuminates the distinctive characteristics of mesenchymal stem cells from various sources, providing insights into their potential applications in regenerative medicine and enhancing our understanding of the inherent diversity of mesenchymal cells in vivo.",
keywords = "allosteric modulators, brain disorders, gpcr, gpcr biased signaling, heterodimers, mglu receptor",
author = "Лобов, {Арсений Андреевич} and Полина Кучур and Хижина, {Айгуль Альфретовна} and Котова, {Анастасия Викторовна} and Андрей Ивашкин and Дарья Костина and Полина Клаузен and Евгения Хохлова and Репкин, {Егор Алексеевич} and Ксения Постникова and Дарья Переплетчикова and Евгений Денисов and Татьяна Геращенко and Рашид Тихилов and Светлана Божкова and Андрей Середа and Виталий Карелкин and Нателла Енукашвили and Малашичева, {Анна Борисовна}",
year = "2023",
month = nov,
day = "1",
doi = "10.1093/stmcls/sxad081",
language = "English",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Mesenchymal cells retain the specificity of embryonal origin during osteogenic differentiation

AU - Лобов, Арсений Андреевич

AU - Кучур , Полина

AU - Хижина, Айгуль Альфретовна

AU - Котова, Анастасия Викторовна

AU - Ивашкин, Андрей

AU - Костина, Дарья

AU - Клаузен, Полина

AU - Хохлова, Евгения

AU - Репкин, Егор Алексеевич

AU - Постникова, Ксения

AU - Переплетчикова , Дарья

AU - Денисов, Евгений

AU - Геращенко , Татьяна

AU - Тихилов, Рашид

AU - Божкова, Светлана

AU - Середа , Андрей

AU - Карелкин, Виталий

AU - Енукашвили , Нателла

AU - Малашичева, Анна Борисовна

PY - 2023/11/1

Y1 - 2023/11/1

N2 - Mesenchymal stem cells (MSCs) are widely used in therapy, but the differences between MSCs of various origins and their ability to undergo osteogenic differentiation and produce extracellular matrix are not fully understood. To address this, we conducted a comparative analysis of mesenchymal cell primary cultures from six human sources, including osteoblast-like cells from the adult femur, adipose-derived stem cells, Wharton's jelly-derived mesenchymal cells, gingival fibroblasts, dental pulp stem cells, and periodontal ligament stem cells. We analyzed these cells' secretome, proteome, and transcriptome under standard and osteogenic cultivation conditions. Despite the overall similarity in osteogenic differentiation, the cells maintain their embryonic specificity after isolation and differentiation in vitro. Furthermore, we propose classifying mesenchymal cells into three groups: dental stem cells of neural crest origin, mesenchymal stem cells, and fetal stem cells. Specifically, fetal stem cells have the most promising secretome for various applications, while mesenchymal stem cells have a specialized secretome optimal for extracellular matrix production. Nevertheless, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. In conclusion, our study illuminates the distinctive characteristics of mesenchymal stem cells from various sources, providing insights into their potential applications in regenerative medicine and enhancing our understanding of the inherent diversity of mesenchymal cells in vivo.

AB - Mesenchymal stem cells (MSCs) are widely used in therapy, but the differences between MSCs of various origins and their ability to undergo osteogenic differentiation and produce extracellular matrix are not fully understood. To address this, we conducted a comparative analysis of mesenchymal cell primary cultures from six human sources, including osteoblast-like cells from the adult femur, adipose-derived stem cells, Wharton's jelly-derived mesenchymal cells, gingival fibroblasts, dental pulp stem cells, and periodontal ligament stem cells. We analyzed these cells' secretome, proteome, and transcriptome under standard and osteogenic cultivation conditions. Despite the overall similarity in osteogenic differentiation, the cells maintain their embryonic specificity after isolation and differentiation in vitro. Furthermore, we propose classifying mesenchymal cells into three groups: dental stem cells of neural crest origin, mesenchymal stem cells, and fetal stem cells. Specifically, fetal stem cells have the most promising secretome for various applications, while mesenchymal stem cells have a specialized secretome optimal for extracellular matrix production. Nevertheless, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. In conclusion, our study illuminates the distinctive characteristics of mesenchymal stem cells from various sources, providing insights into their potential applications in regenerative medicine and enhancing our understanding of the inherent diversity of mesenchymal cells in vivo.

KW - allosteric modulators

KW - brain disorders

KW - gpcr

KW - gpcr biased signaling

KW - heterodimers

KW - mglu receptor

UR - https://academic.oup.com/stmcls/advance-article/doi/10.1093/stmcls/sxad081/7336816

UR - https://www.mendeley.com/catalogue/49adcdc8-1dfb-33dd-bdc6-bdf1b972f813/

U2 - 10.1093/stmcls/sxad081

DO - 10.1093/stmcls/sxad081

M3 - Article

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

M1 - sxad081

ER -

ID: 113871097