TY - JOUR

T1 - Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines

AU - Sokolov, A.V.

AU - Kostevich, V.A.

AU - Kozlov, S.O.

AU - Donskyi, I.S.

AU - Vlasova, I.I.

AU - Rudenko, A.O.

AU - Zakharova, E.T.

AU - Vasilyev, V.B.

AU - Panasenko, O.M.

PY - 2015

Y1 - 2015

N2 - Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a substrate for the peroxidase activity of MPO and does not react with hydrogen peroxide and superoxide anion radical. Taurine N-chloramine (bromamine) reacts with CB in molar ratio of 1:2. Using the new method, we studied the dependence of MPO activity on concentration of substrates and inhibitors. The specificity of MPO inhibition by non-proteolyzed ceruloplasmin is characterized. The inhibition of taurine N-chloramine production by neutrophils and HL-60 cells in the presence of MPO-affecting substances i

AB - Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a substrate for the peroxidase activity of MPO and does not react with hydrogen peroxide and superoxide anion radical. Taurine N-chloramine (bromamine) reacts with CB in molar ratio of 1:2. Using the new method, we studied the dependence of MPO activity on concentration of substrates and inhibitors. The specificity of MPO inhibition by non-proteolyzed ceruloplasmin is characterized. The inhibition of taurine N-chloramine production by neutrophils and HL-60 cells in the presence of MPO-affecting substances i

KW - HOCl

KW - celestine blue B

KW - ceruloplasmin

KW - halogenating activity

KW - myeloperoxidase

KW - taurine

U2 - 10.3109/10715762.2015.1017478

DO - 10.3109/10715762.2015.1017478

M3 - Article

VL - 49

SP - 777

EP - 789

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 6

ER -