Standard

Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines. / Sokolov, A.V.; Kostevich, V.A.; Kozlov, S.O.; Donskyi, I.S.; Vlasova, I.I.; Rudenko, A.O.; Zakharova, E.T.; Vasilyev, V.B.; Panasenko, O.M.

In: Free Radical Research, Vol. 49, No. 6, 2015, p. 777-789.

Research output: Contribution to journal › Article

Harvard

Sokolov, AV, Kostevich, VA, Kozlov, SO, Donskyi, IS, Vlasova, II, Rudenko, AO, Zakharova, ET, Vasilyev, VB & Panasenko, OM 2015, 'Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines', Free Radical Research, vol. 49, no. 6, pp. 777-789. https://doi.org/10.3109/10715762.2015.1017478

APA

Sokolov, A. V., Kostevich, V. A., Kozlov, S. O., Donskyi, I. S., Vlasova, I. I., Rudenko, A. O., Zakharova, E. T., Vasilyev, V. B., & Panasenko, O. M. (2015). Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines. Free Radical Research, 49(6), 777-789. https://doi.org/10.3109/10715762.2015.1017478

Vancouver

Sokolov AV, Kostevich VA, Kozlov SO, Donskyi IS, Vlasova II, Rudenko AO et al. Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines. Free Radical Research. 2015;49(6):777-789. https://doi.org/10.3109/10715762.2015.1017478

Author

Sokolov, A.V. ; Kostevich, V.A. ; Kozlov, S.O. ; Donskyi, I.S. ; Vlasova, I.I. ; Rudenko, A.O. ; Zakharova, E.T. ; Vasilyev, V.B. ; Panasenko, O.M. / Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines. In: Free Radical Research. 2015 ; Vol. 49, No. 6. pp. 777-789.

BibTeX

@article{b72e8ed290ae43959da55b3feff227af,
title = "Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines",
abstract = "Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a substrate for the peroxidase activity of MPO and does not react with hydrogen peroxide and superoxide anion radical. Taurine N-chloramine (bromamine) reacts with CB in molar ratio of 1:2. Using the new method, we studied the dependence of MPO activity on concentration of substrates and inhibitors. The specificity of MPO inhibition by non-proteolyzed ceruloplasmin is characterized. The inhibition of taurine N-chloramine production by neutrophils and HL-60 cells in the presence of MPO-affecting substances i",
keywords = "HOCl, celestine blue B, ceruloplasmin, halogenating activity, myeloperoxidase, taurine",
author = "A.V. Sokolov and V.A. Kostevich and S.O. Kozlov and I.S. Donskyi and I.I. Vlasova and A.O. Rudenko and E.T. Zakharova and V.B. Vasilyev and O.M. Panasenko",
year = "2015",
doi = "10.3109/10715762.2015.1017478",
language = "English",
volume = "49",
pages = "777--789",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Informa Healthcare",
number = "6",

}

RIS

TY - JOUR

T1 - Kinetic method for assaying the halogenating activity of myeloperoxidase based on reaction of celestine blue B with taurine halogenamines

AU - Sokolov, A.V.

AU - Kostevich, V.A.

AU - Kozlov, S.O.

AU - Donskyi, I.S.

AU - Vlasova, I.I.

AU - Rudenko, A.O.

AU - Zakharova, E.T.

AU - Vasilyev, V.B.

AU - Panasenko, O.M.

PY - 2015

Y1 - 2015

N2 - Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a substrate for the peroxidase activity of MPO and does not react with hydrogen peroxide and superoxide anion radical. Taurine N-chloramine (bromamine) reacts with CB in molar ratio of 1:2. Using the new method, we studied the dependence of MPO activity on concentration of substrates and inhibitors. The specificity of MPO inhibition by non-proteolyzed ceruloplasmin is characterized. The inhibition of taurine N-chloramine production by neutrophils and HL-60 cells in the presence of MPO-affecting substances i

AB - Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a substrate for the peroxidase activity of MPO and does not react with hydrogen peroxide and superoxide anion radical. Taurine N-chloramine (bromamine) reacts with CB in molar ratio of 1:2. Using the new method, we studied the dependence of MPO activity on concentration of substrates and inhibitors. The specificity of MPO inhibition by non-proteolyzed ceruloplasmin is characterized. The inhibition of taurine N-chloramine production by neutrophils and HL-60 cells in the presence of MPO-affecting substances i

KW - HOCl

KW - celestine blue B

KW - ceruloplasmin

KW - halogenating activity

KW - myeloperoxidase

KW - taurine

U2 - 10.3109/10715762.2015.1017478

DO - 10.3109/10715762.2015.1017478

M3 - Article

VL - 49

SP - 777

EP - 789

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 6

ER -

ID: 3937833