Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting. / Dedukh, Dmitrij; Maslova, Antonina; Al-Rikabi, Ahmed; Padutsch, Niklas; Liehr, Thomas; Krasikova, Alla.
в: Chromosoma, Том 132, № 4, 11.2023, стр. 329-342.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting
AU - Dedukh, Dmitrij
AU - Maslova, Antonina
AU - Al-Rikabi, Ahmed
AU - Padutsch, Niklas
AU - Liehr, Thomas
AU - Krasikova, Alla
N1 - © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2023/11
Y1 - 2023/11
N2 - Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.
AB - Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.
KW - Chromosomal painting
KW - Chromosome evolution
KW - Clonal hybrids
KW - Cytogenetics
KW - Fluorescence in situ hybridization
KW - Pelophylax esculentus complex
KW - Anura/genetics
KW - Chromosome Painting
KW - Ranidae/genetics
KW - Animals
KW - Karyotyping
KW - Karyotype
KW - Rana esculenta/genetics
UR - https://www.mendeley.com/catalogue/ec4db0cb-d35d-31a0-a290-5aaf54dcf986/
U2 - 10.1007/s00412-023-00812-8
DO - 10.1007/s00412-023-00812-8
M3 - Article
C2 - 38001396
VL - 132
SP - 329
EP - 342
JO - Chromosoma
JF - Chromosoma
SN - 0009-5915
IS - 4
ER -
ID: 114374987