Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting

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Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting. / Dedukh, Dmitrij; Maslova, Antonina; Al-Rikabi, Ahmed; Padutsch, Niklas; Liehr, Thomas; Krasikova, Alla.

In: Chromosoma, Vol. 132, No. 4, 11.2023, p. 329-342.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{600f49dd33864496959bbad03e6bb205,

title = "Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting",

abstract = "Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.",

keywords = "Chromosomal painting, Chromosome evolution, Clonal hybrids, Cytogenetics, Fluorescence in situ hybridization, Pelophylax esculentus complex, Anura/genetics, Chromosome Painting, Ranidae/genetics, Animals, Karyotyping, Karyotype, Rana esculenta/genetics",

author = "Dmitrij Dedukh and Antonina Maslova and Ahmed Al-Rikabi and Niklas Padutsch and Thomas Liehr and Alla Krasikova",

note = "{\textcopyright} 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2023",

month = nov,

doi = "10.1007/s00412-023-00812-8",

language = "English",

volume = "132",

pages = "329--342",

journal = "Chromosoma",

issn = "0009-5915",

publisher = "Springer Nature",

number = "4",

}

RIS

TY - JOUR

T1 - Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting

AU - Dedukh, Dmitrij

AU - Maslova, Antonina

AU - Al-Rikabi, Ahmed

AU - Padutsch, Niklas

AU - Liehr, Thomas

AU - Krasikova, Alla

PY - 2023/11

Y1 - 2023/11

N2 - Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.

AB - Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.

KW - Chromosomal painting

KW - Chromosome evolution

KW - Clonal hybrids

KW - Cytogenetics

KW - Fluorescence in situ hybridization

KW - Pelophylax esculentus complex

KW - Anura/genetics

KW - Chromosome Painting

KW - Ranidae/genetics

KW - Animals

KW - Karyotyping

KW - Karyotype

KW - Rana esculenta/genetics

UR - https://www.mendeley.com/catalogue/ec4db0cb-d35d-31a0-a290-5aaf54dcf986/

U2 - 10.1007/s00412-023-00812-8

DO - 10.1007/s00412-023-00812-8

M3 - Article

C2 - 38001396

VL - 132

SP - 329

EP - 342

JO - Chromosoma

JF - Chromosoma

SN - 0009-5915

IS - 4

ER -

ID: 114374987