TY - JOUR

T1 - Increasing the legume–rhizobia symbiotic efficiency due to the synergy between commercial strains and strains isolated from relict symbiotic systems

AU - Safronova, Vera I.

AU - Sazanova, Anna L.

AU - Kuznetsova, Irina G.

AU - Belimov, Andrey A.

AU - Guro , Polina

AU - Karlov, Denis S.

AU - Yuzikhin, Oleg S.

AU - Chirak, Elizaveta R.

AU - Verkhozina, Alla

AU - Afonin, Alexey A.

AU - Andronov, Evgeny

AU - Tikhonovich, Igor

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/7

Y1 - 2021/7

N2 - The phenomenon of rhizobial synergy was investigated to increase the efficiency of nitrogen-fixing symbiosis of alfalfa (Medicago varia Martyn), common vetch (Vicia sativa L.) or red clover (Trifolium pratense L.). These plants were co-inoculated with the respective commercial strains Sinorhizobium meliloti RCAM1750, Rhizobium leguminosarum RCAM0626 or R. leguminosarum RCAM1365 and with the strains Mesorhizobium japonicum Opo-235, M. japonicum Opo-242, Bradyrhizobium sp. Opo-243 or M. kowhaii Ach-343 isolated from the relict legumes Oxytropis popoviana Peschkova and Astragalus chorinensis Bunge. The isolates mentioned above had additional symbiotic genes (fix, nif, nod, noe and nol) as well as the genes promoting plant growth and symbiosis formation (acdRS, genes associated with the biosynthesis of gibberellins and auxins, genes of T3SS, T4SS and T6SS secretion systems) compared to the commercial strains. Nodulation assays showed that in some variants of co-inoculation the symbiotic parameters of plants such as nodule number, plant biomass or acetylene reduction activity were increased. We assume that the study of microbial synergy using rhizobia of relict legumes will make it possible to carry out targeted selection of co-microsymbionts to increase the efficiency of agricultural legume–rhizobia systems.

AB - The phenomenon of rhizobial synergy was investigated to increase the efficiency of nitrogen-fixing symbiosis of alfalfa (Medicago varia Martyn), common vetch (Vicia sativa L.) or red clover (Trifolium pratense L.). These plants were co-inoculated with the respective commercial strains Sinorhizobium meliloti RCAM1750, Rhizobium leguminosarum RCAM0626 or R. leguminosarum RCAM1365 and with the strains Mesorhizobium japonicum Opo-235, M. japonicum Opo-242, Bradyrhizobium sp. Opo-243 or M. kowhaii Ach-343 isolated from the relict legumes Oxytropis popoviana Peschkova and Astragalus chorinensis Bunge. The isolates mentioned above had additional symbiotic genes (fix, nif, nod, noe and nol) as well as the genes promoting plant growth and symbiosis formation (acdRS, genes associated with the biosynthesis of gibberellins and auxins, genes of T3SS, T4SS and T6SS secretion systems) compared to the commercial strains. Nodulation assays showed that in some variants of co-inoculation the symbiotic parameters of plants such as nodule number, plant biomass or acetylene reduction activity were increased. We assume that the study of microbial synergy using rhizobia of relict legumes will make it possible to carry out targeted selection of co-microsymbionts to increase the efficiency of agricultural legume–rhizobia systems.

KW - plant-microbe interactions

KW - crop legumes

KW - relict symbiotic systems

KW - rhizobial synergy

KW - efficiency of symbiosis

KW - Plant-microbe interaction

KW - Crop legumes

KW - Relict symbiotic systems

KW - Rhizobial synergy

KW - Efficiency of symbiosis

UR - https://www.mdpi.com/2073-4395/11/7/1398

UR - http://www.scopus.com/inward/record.url?scp=85111058952&partnerID=8YFLogxK

U2 - 10.3390/agronomy11071398

DO - 10.3390/agronomy11071398

M3 - Article

VL - 11

JO - Agronomy

JF - Agronomy

SN - 2073-4395

IS - 7

M1 - 1398

ER -