Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Hyaluronan/colistin polyelectrolyte complexes : Promising antiinfective drug delivery systems. / Dubashynskaya, Natallia V.; Raik, Sergei V.; Dubrovskii, Yaroslav A.; Shcherbakova, Elena S.; Demyanova, Elena V.; Shasherina, Anna Y.; Anufrikov, Yuri A.; Poshina, Daria N.; Dobrodumov, Anatoliy V.; Skorik, Yury A.
в: International Journal of Biological Macromolecules, Том 187, 30.09.2021, стр. 157-165.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Hyaluronan/colistin polyelectrolyte complexes
T2 - Promising antiinfective drug delivery systems
AU - Dubashynskaya, Natallia V.
AU - Raik, Sergei V.
AU - Dubrovskii, Yaroslav A.
AU - Shcherbakova, Elena S.
AU - Demyanova, Elena V.
AU - Shasherina, Anna Y.
AU - Anufrikov, Yuri A.
AU - Poshina, Daria N.
AU - Dobrodumov, Anatoliy V.
AU - Skorik, Yury A.
N1 - Publisher Copyright: © 2021 Elsevier B.V.
PY - 2021/9/30
Y1 - 2021/9/30
N2 - Nanotechnology-based modification of known antimicrobial agents is a rational and straightforward way to improve their safety and effectiveness. The aim of this study was to develop colistin (CT)-loaded polymeric carriers based on hyaluronic acid (HA) for potential application as antimicrobial agents against multi-resistant gram-negative microorganisms (including ESKAPE pathogens). CT-containing particles were obtained via a polyelectrolyte interaction between protonated CT amino groups and HA carboxyl groups (the CT-HA complex formation constant [logKCT-HA] was about 5.0). The resulting polyelectrolyte complexes had a size of 210–250 nm and a negative charge (ζ-potential −19 mV), with encapsulation and loading efficiencies of 100% and 20%, respectively. The developed CT delivery systems were characterized by modified release (45% and 85% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro tests showed that the encapsulation of CT in polymer particles did not reduce its pharmacological activity; the minimum inhibitory concentrations of both encapsulated CT and pure CT were 1 μg/mL (against Pseudomonas aeruginosa).
AB - Nanotechnology-based modification of known antimicrobial agents is a rational and straightforward way to improve their safety and effectiveness. The aim of this study was to develop colistin (CT)-loaded polymeric carriers based on hyaluronic acid (HA) for potential application as antimicrobial agents against multi-resistant gram-negative microorganisms (including ESKAPE pathogens). CT-containing particles were obtained via a polyelectrolyte interaction between protonated CT amino groups and HA carboxyl groups (the CT-HA complex formation constant [logKCT-HA] was about 5.0). The resulting polyelectrolyte complexes had a size of 210–250 nm and a negative charge (ζ-potential −19 mV), with encapsulation and loading efficiencies of 100% and 20%, respectively. The developed CT delivery systems were characterized by modified release (45% and 85% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro tests showed that the encapsulation of CT in polymer particles did not reduce its pharmacological activity; the minimum inhibitory concentrations of both encapsulated CT and pure CT were 1 μg/mL (against Pseudomonas aeruginosa).
KW - Colistin, Hyaluronic acid, drug delivery system
KW - Colistin
KW - Drug delivery system
KW - Hyaluronic acid
KW - ANTIBIOTICS
KW - INFECTIONS
KW - POLYMYXINS
KW - NANOCARRIERS
KW - HYALURONIC-ACID
KW - FUNCTIONALIZATION
KW - NANOPARTICLES
KW - PEPTIDES
KW - COLISTIN
KW - RESISTANCE
UR - https://proxy.library.spbu.ru:2068/science/article/pii/S0141813021015646?via%3Dihub
UR - http://www.scopus.com/inward/record.url?scp=85111059423&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/e1f01331-55c9-39f3-92d0-37be93d8e938/
U2 - 10.1016/j.ijbiomac.2021.07.114
DO - 10.1016/j.ijbiomac.2021.07.114
M3 - Article
VL - 187
SP - 157
EP - 165
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -
ID: 87285952