TY - JOUR

T1 - Hyaluronan/colistin polyelectrolyte complexes

T2 - Promising antiinfective drug delivery systems

AU - Dubashynskaya, Natallia V.

AU - Raik, Sergei V.

AU - Dubrovskii, Yaroslav A.

AU - Shcherbakova, Elena S.

AU - Demyanova, Elena V.

AU - Shasherina, Anna Y.

AU - Anufrikov, Yuri A.

AU - Poshina, Daria N.

AU - Dobrodumov, Anatoliy V.

AU - Skorik, Yury A.

N1 - Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/9/30

Y1 - 2021/9/30

N2 - Nanotechnology-based modification of known antimicrobial agents is a rational and straightforward way to improve their safety and effectiveness. The aim of this study was to develop colistin (CT)-loaded polymeric carriers based on hyaluronic acid (HA) for potential application as antimicrobial agents against multi-resistant gram-negative microorganisms (including ESKAPE pathogens). CT-containing particles were obtained via a polyelectrolyte interaction between protonated CT amino groups and HA carboxyl groups (the CT-HA complex formation constant [logKCT-HA] was about 5.0). The resulting polyelectrolyte complexes had a size of 210–250 nm and a negative charge (ζ-potential −19 mV), with encapsulation and loading efficiencies of 100% and 20%, respectively. The developed CT delivery systems were characterized by modified release (45% and 85% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro tests showed that the encapsulation of CT in polymer particles did not reduce its pharmacological activity; the minimum inhibitory concentrations of both encapsulated CT and pure CT were 1 μg/mL (against Pseudomonas aeruginosa).

AB - Nanotechnology-based modification of known antimicrobial agents is a rational and straightforward way to improve their safety and effectiveness. The aim of this study was to develop colistin (CT)-loaded polymeric carriers based on hyaluronic acid (HA) for potential application as antimicrobial agents against multi-resistant gram-negative microorganisms (including ESKAPE pathogens). CT-containing particles were obtained via a polyelectrolyte interaction between protonated CT amino groups and HA carboxyl groups (the CT-HA complex formation constant [logKCT-HA] was about 5.0). The resulting polyelectrolyte complexes had a size of 210–250 nm and a negative charge (ζ-potential −19 mV), with encapsulation and loading efficiencies of 100% and 20%, respectively. The developed CT delivery systems were characterized by modified release (45% and 85% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro tests showed that the encapsulation of CT in polymer particles did not reduce its pharmacological activity; the minimum inhibitory concentrations of both encapsulated CT and pure CT were 1 μg/mL (against Pseudomonas aeruginosa).

KW - Colistin, Hyaluronic acid, drug delivery system

KW - Colistin

KW - Drug delivery system

KW - Hyaluronic acid

KW - ANTIBIOTICS

KW - INFECTIONS

KW - POLYMYXINS

KW - NANOCARRIERS

KW - HYALURONIC-ACID

KW - FUNCTIONALIZATION

KW - NANOPARTICLES

KW - PEPTIDES

KW - COLISTIN

KW - RESISTANCE

UR - https://proxy.library.spbu.ru:2068/science/article/pii/S0141813021015646?via%3Dihub

UR - http://www.scopus.com/inward/record.url?scp=85111059423&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/e1f01331-55c9-39f3-92d0-37be93d8e938/

U2 - 10.1016/j.ijbiomac.2021.07.114

DO - 10.1016/j.ijbiomac.2021.07.114

M3 - Article

VL - 187

SP - 157

EP - 165

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -