Homogeneous liquid-liquid microextraction based on primary amine phase separation: a novel approach for sample pretreatment

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Homogeneous liquid-liquid microextraction based on primary amine phase separation: a novel approach for sample pretreatment. / Cherkashina, Ksenia; Lebedinets, Sofya ; Pochivalov, Aleksei ; Lezov, Aleksey ; Vakh, Christina ; Bulatov, Andrey.

в: Analytica Chimica Acta, Том 1074, 2019, стр. 117-122.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{53f7a6568bdb498b8cb9d65e61b60e8a,

title = "Homogeneous liquid-liquid microextraction based on primary amine phase separation: a novel approach for sample pretreatment",

abstract = "A homogeneous liquid-liquid microextraction procedure based on primary amine phase separation was developed as a novel approach for the pretreatment of biological fluids. The procedure assumes primary amine dissolution in the aqueous sample phase, resulting in the creation of a homogeneous sample solution followed by phase separation and analyte extraction in the presence of a polar, water-miscible organic solvent. The phenomenon of primary amine phase separation from homogeneous aqueous solution in the presence of a polar, water-miscible organic solvent was studied in detail and applied for separation and preconcentration in chemical analysis for the first time. The suggested approach was used for the HPLC-UV determination of meropenem as a proof-of-concept analyte in human plasma and serum samples. Under optimal conditions, the detector response of meropenem was linear in the concentration range of 0.1–100.0 mg L −1. The limit of detection was calculated from a blank test based on 3σ, was 0.03 mg L −1. ",

keywords = "Biological fluids, HPLC-UV, Homogeneous liquid-liquid microextraction, Meropenem, Primary amine",

author = "Ksenia Cherkashina and Sofya Lebedinets and Aleksei Pochivalov and Aleksey Lezov and Christina Vakh and Andrey Bulatov",

year = "2019",

doi = "10.1016/j.aca.2019.04.070",

language = "English",

volume = "1074",

pages = "117--122",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Homogeneous liquid-liquid microextraction based on primary amine phase separation: a novel approach for sample pretreatment

AU - Cherkashina, Ksenia

AU - Lebedinets, Sofya

AU - Pochivalov, Aleksei

AU - Lezov, Aleksey

AU - Vakh, Christina

AU - Bulatov, Andrey

PY - 2019

Y1 - 2019

N2 - A homogeneous liquid-liquid microextraction procedure based on primary amine phase separation was developed as a novel approach for the pretreatment of biological fluids. The procedure assumes primary amine dissolution in the aqueous sample phase, resulting in the creation of a homogeneous sample solution followed by phase separation and analyte extraction in the presence of a polar, water-miscible organic solvent. The phenomenon of primary amine phase separation from homogeneous aqueous solution in the presence of a polar, water-miscible organic solvent was studied in detail and applied for separation and preconcentration in chemical analysis for the first time. The suggested approach was used for the HPLC-UV determination of meropenem as a proof-of-concept analyte in human plasma and serum samples. Under optimal conditions, the detector response of meropenem was linear in the concentration range of 0.1–100.0 mg L −1. The limit of detection was calculated from a blank test based on 3σ, was 0.03 mg L −1.

AB - A homogeneous liquid-liquid microextraction procedure based on primary amine phase separation was developed as a novel approach for the pretreatment of biological fluids. The procedure assumes primary amine dissolution in the aqueous sample phase, resulting in the creation of a homogeneous sample solution followed by phase separation and analyte extraction in the presence of a polar, water-miscible organic solvent. The phenomenon of primary amine phase separation from homogeneous aqueous solution in the presence of a polar, water-miscible organic solvent was studied in detail and applied for separation and preconcentration in chemical analysis for the first time. The suggested approach was used for the HPLC-UV determination of meropenem as a proof-of-concept analyte in human plasma and serum samples. Under optimal conditions, the detector response of meropenem was linear in the concentration range of 0.1–100.0 mg L −1. The limit of detection was calculated from a blank test based on 3σ, was 0.03 mg L −1.

KW - Biological fluids

KW - HPLC-UV

KW - Homogeneous liquid-liquid microextraction

KW - Meropenem

KW - Primary amine

UR - http://www.scopus.com/inward/record.url?scp=85066084495&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2019.04.070

DO - 10.1016/j.aca.2019.04.070

M3 - Article

VL - 1074

SP - 117

EP - 122

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

ER -

ID: 41413016