Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
A homogeneous liquid-liquid microextraction procedure based on primary amine phase separation was developed as a novel approach for the pretreatment of biological fluids. The procedure assumes primary amine dissolution in the aqueous sample phase, resulting in the creation of a homogeneous sample solution followed by phase separation and analyte extraction in the presence of a polar, water-miscible organic solvent. The phenomenon of primary amine phase separation from homogeneous aqueous solution in the presence of a polar, water-miscible organic solvent was studied in detail and applied for separation and preconcentration in chemical analysis for the first time. The suggested approach was used for the HPLC-UV determination of meropenem as a proof-of-concept analyte in human plasma and serum samples. Under optimal conditions, the detector response of meropenem was linear in the concentration range of 0.1–100.0 mg L −1. The limit of detection was calculated from a blank test based on 3σ, was 0.03 mg L −1.
Язык оригинала | английский |
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Страницы (с-по) | 117-122 |
Число страниц | 6 |
Журнал | Analytica Chimica Acta |
Том | 1074 |
DOI | |
Состояние | Опубликовано - 2019 |
ID: 41413016