High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium

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High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium. / Plotnikov, Andrey O.; Balkin, Alexander S.; Gogoleva, Natalia E.; Lanzoni, Olivia; Khlopko, Yuri A.; Cherkasov, Sergey V.; Potekhin, Alexey A.

в: Microbial Ecology, Том 78, № 2, 15.08.2019, стр. 286-298.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Author

Plotnikov, Andrey O. ; Balkin, Alexander S. ; Gogoleva, Natalia E. ; Lanzoni, Olivia ; Khlopko, Yuri A. ; Cherkasov, Sergey V. ; Potekhin, Alexey A. / High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium. в: Microbial Ecology. 2019 ; Том 78, № 2. стр. 286-298.

BibTeX

@article{323ac6881b5a48cc9db3bed2f506e9c0,

title = "High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium",

abstract = "Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.",

keywords = "Bacterial communities, Ciliates, Human pathogens and commensals, Metabarcoding, Microbiomes, Single cell sequencing, BACTERIAL COMMUNITY, PREDATION, YANTAIENSIS, WATER-COLUMN, SINGLE-CELL, MYCOBACTERIA, AMEBAS, PROTOZOA, PATHOGENS, PROKARYOTES",

author = "Plotnikov, {Andrey O.} and Balkin, {Alexander S.} and Gogoleva, {Natalia E.} and Olivia Lanzoni and Khlopko, {Yuri A.} and Cherkasov, {Sergey V.} and Potekhin, {Alexey A.}",

year = "2019",

month = aug,

day = "15",

doi = "10.1007/s00248-019-01321-x",

language = "English",

volume = "78",

pages = "286--298",

journal = "Microbial Ecology",

issn = "0095-3628",

publisher = "Springer Nature",

number = "2",

}

RIS

TY - JOUR

T1 - High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium

AU - Plotnikov, Andrey O.

AU - Balkin, Alexander S.

AU - Gogoleva, Natalia E.

AU - Lanzoni, Olivia

AU - Khlopko, Yuri A.

AU - Cherkasov, Sergey V.

AU - Potekhin, Alexey A.

PY - 2019/8/15

Y1 - 2019/8/15

N2 - Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.

AB - Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.

KW - Bacterial communities

KW - Ciliates

KW - Human pathogens and commensals

KW - Metabarcoding

KW - Microbiomes

KW - Single cell sequencing

KW - BACTERIAL COMMUNITY

KW - PREDATION

KW - YANTAIENSIS

KW - WATER-COLUMN

KW - SINGLE-CELL

KW - MYCOBACTERIA

KW - AMEBAS

KW - PROTOZOA

KW - PATHOGENS

KW - PROKARYOTES

UR - http://www.scopus.com/inward/record.url?scp=85060340590&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/highthroughput-sequencing-16s-rrna-gene-survey-analyze-microbiomes-freeliving-ciliates-paramecium

U2 - 10.1007/s00248-019-01321-x

DO - 10.1007/s00248-019-01321-x

M3 - Article

AN - SCOPUS:85060340590

VL - 78

SP - 286

EP - 298

JO - Microbial Ecology

JF - Microbial Ecology

SN - 0095-3628

IS - 2

ER -

ID: 39764609