Research output: Contribution to journal › Article › peer-review
High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium. / Plotnikov, Andrey O.; Balkin, Alexander S.; Gogoleva, Natalia E.; Lanzoni, Olivia; Khlopko, Yuri A.; Cherkasov, Sergey V.; Potekhin, Alexey A.
In: Microbial Ecology, Vol. 78, No. 2, 15.08.2019, p. 286-298.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium
AU - Plotnikov, Andrey O.
AU - Balkin, Alexander S.
AU - Gogoleva, Natalia E.
AU - Lanzoni, Olivia
AU - Khlopko, Yuri A.
AU - Cherkasov, Sergey V.
AU - Potekhin, Alexey A.
PY - 2019/8/15
Y1 - 2019/8/15
N2 - Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.
AB - Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.
KW - Bacterial communities
KW - Ciliates
KW - Human pathogens and commensals
KW - Metabarcoding
KW - Microbiomes
KW - Single cell sequencing
KW - BACTERIAL COMMUNITY
KW - PREDATION
KW - YANTAIENSIS
KW - WATER-COLUMN
KW - SINGLE-CELL
KW - MYCOBACTERIA
KW - AMEBAS
KW - PROTOZOA
KW - PATHOGENS
KW - PROKARYOTES
UR - http://www.scopus.com/inward/record.url?scp=85060340590&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/highthroughput-sequencing-16s-rrna-gene-survey-analyze-microbiomes-freeliving-ciliates-paramecium
U2 - 10.1007/s00248-019-01321-x
DO - 10.1007/s00248-019-01321-x
M3 - Article
AN - SCOPUS:85060340590
VL - 78
SP - 286
EP - 298
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 2
ER -
ID: 39764609