TY - JOUR

T1 - Genetic control of in vitro differentiation processes in radish

AU - Lutova, L. A.

AU - Buzovkina, I. S.

AU - Smirnova, O. A.

AU - Tikhodeyev, O. N.

AU - Shishkova, S. O.

AU - Trifonova, I. M.

N1 - Funding Information: The present work was supported by the Russian Fund for Fundamental In~,estigations, International Soros Science Program and Civilian Research and Development Foundation.

PY - 1997

Y1 - 1997

N2 - Genetic control of differentiation processes in radish was studied in vitro on the level of morphogenic capacities of explants. We have shown that when cultured on hormone-free MS medium (Murashige and Skoog, 1962), isolated radish cotyledons can produce callus and/or roots. At the same time, excised seedling apices placed on MS medium supplied with exogenous cytokinin can form multiple shoots or crop-root-like structures. In our model, the ability of explants to undergo the above morphogenic events in culture under certain in vitro conditions was examined as a genetic marker. As forms tested, highly inbred radish lines maintained by tight inbreeding for 28-34 generations were used. We have shown that ability of excised cotyledons to produce callus is controlled by a single gene, while their root-producing capacity is under di-genic control with some additional influence of the cytoplasm. Analysis of inheritance of seedling apex capacity to produce crop-root-like structures in response to exogenous cytokinin led us to propose the interaction of three genes in control of this trait.

AB - Genetic control of differentiation processes in radish was studied in vitro on the level of morphogenic capacities of explants. We have shown that when cultured on hormone-free MS medium (Murashige and Skoog, 1962), isolated radish cotyledons can produce callus and/or roots. At the same time, excised seedling apices placed on MS medium supplied with exogenous cytokinin can form multiple shoots or crop-root-like structures. In our model, the ability of explants to undergo the above morphogenic events in culture under certain in vitro conditions was examined as a genetic marker. As forms tested, highly inbred radish lines maintained by tight inbreeding for 28-34 generations were used. We have shown that ability of excised cotyledons to produce callus is controlled by a single gene, while their root-producing capacity is under di-genic control with some additional influence of the cytoplasm. Analysis of inheritance of seedling apex capacity to produce crop-root-like structures in response to exogenous cytokinin led us to propose the interaction of three genes in control of this trait.

KW - Cytokinin

KW - Explant sensitivity

KW - In vitro differentiation

KW - Inbred lines

KW - Phytohormone variation

UR - http://www.scopus.com/inward/record.url?scp=0031462076&partnerID=8YFLogxK

U2 - 10.1007/s11627-997-0048-0

DO - 10.1007/s11627-997-0048-0

M3 - Article

AN - SCOPUS:0031462076

VL - 33

SP - 269

EP - 274

JO - In Vitro

JF - In Vitro

SN - 0073-5655

IS - 4

ER -