Genetic control of in vitro differentiation processes in radish

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Genetic control of in vitro differentiation processes in radish. / Lutova, L. A.; Buzovkina, I. S.; Smirnova, O. A.; Tikhodeyev, O. N.; Shishkova, S. O.; Trifonova, I. M.

In: In Vitro Cellular and Developmental Biology - Plant, Vol. 33, No. 4, 1997, p. 269-274.

Research output: Contribution to journal › Article › peer-review

Author

Lutova, L. A. ; Buzovkina, I. S. ; Smirnova, O. A. ; Tikhodeyev, O. N. ; Shishkova, S. O. ; Trifonova, I. M. / Genetic control of in vitro differentiation processes in radish. In: In Vitro Cellular and Developmental Biology - Plant. 1997 ; Vol. 33, No. 4. pp. 269-274.

BibTeX

@article{cec960e9107a45de905ee42e707d29af,

title = "Genetic control of in vitro differentiation processes in radish",

abstract = "Genetic control of differentiation processes in radish was studied in vitro on the level of morphogenic capacities of explants. We have shown that when cultured on hormone-free MS medium (Murashige and Skoog, 1962), isolated radish cotyledons can produce callus and/or roots. At the same time, excised seedling apices placed on MS medium supplied with exogenous cytokinin can form multiple shoots or crop-root-like structures. In our model, the ability of explants to undergo the above morphogenic events in culture under certain in vitro conditions was examined as a genetic marker. As forms tested, highly inbred radish lines maintained by tight inbreeding for 28-34 generations were used. We have shown that ability of excised cotyledons to produce callus is controlled by a single gene, while their root-producing capacity is under di-genic control with some additional influence of the cytoplasm. Analysis of inheritance of seedling apex capacity to produce crop-root-like structures in response to exogenous cytokinin led us to propose the interaction of three genes in control of this trait.",

keywords = "Cytokinin, Explant sensitivity, In vitro differentiation, Inbred lines, Phytohormone variation",

author = "Lutova, {L. A.} and Buzovkina, {I. S.} and Smirnova, {O. A.} and Tikhodeyev, {O. N.} and Shishkova, {S. O.} and Trifonova, {I. M.}",

note = "Funding Information: The present work was supported by the Russian Fund for Fundamental In~,estigations, International Soros Science Program and Civilian Research and Development Foundation.",

year = "1997",

doi = "10.1007/s11627-997-0048-0",

language = "English",

volume = "33",

pages = "269--274",

journal = "In Vitro",

issn = "0073-5655",

publisher = "Springer Nature",

number = "4",

}

RIS

TY - JOUR

T1 - Genetic control of in vitro differentiation processes in radish

AU - Lutova, L. A.

AU - Buzovkina, I. S.

AU - Smirnova, O. A.

AU - Tikhodeyev, O. N.

AU - Shishkova, S. O.

AU - Trifonova, I. M.

N1 - Funding Information: The present work was supported by the Russian Fund for Fundamental In~,estigations, International Soros Science Program and Civilian Research and Development Foundation.

PY - 1997

Y1 - 1997

N2 - Genetic control of differentiation processes in radish was studied in vitro on the level of morphogenic capacities of explants. We have shown that when cultured on hormone-free MS medium (Murashige and Skoog, 1962), isolated radish cotyledons can produce callus and/or roots. At the same time, excised seedling apices placed on MS medium supplied with exogenous cytokinin can form multiple shoots or crop-root-like structures. In our model, the ability of explants to undergo the above morphogenic events in culture under certain in vitro conditions was examined as a genetic marker. As forms tested, highly inbred radish lines maintained by tight inbreeding for 28-34 generations were used. We have shown that ability of excised cotyledons to produce callus is controlled by a single gene, while their root-producing capacity is under di-genic control with some additional influence of the cytoplasm. Analysis of inheritance of seedling apex capacity to produce crop-root-like structures in response to exogenous cytokinin led us to propose the interaction of three genes in control of this trait.

AB - Genetic control of differentiation processes in radish was studied in vitro on the level of morphogenic capacities of explants. We have shown that when cultured on hormone-free MS medium (Murashige and Skoog, 1962), isolated radish cotyledons can produce callus and/or roots. At the same time, excised seedling apices placed on MS medium supplied with exogenous cytokinin can form multiple shoots or crop-root-like structures. In our model, the ability of explants to undergo the above morphogenic events in culture under certain in vitro conditions was examined as a genetic marker. As forms tested, highly inbred radish lines maintained by tight inbreeding for 28-34 generations were used. We have shown that ability of excised cotyledons to produce callus is controlled by a single gene, while their root-producing capacity is under di-genic control with some additional influence of the cytoplasm. Analysis of inheritance of seedling apex capacity to produce crop-root-like structures in response to exogenous cytokinin led us to propose the interaction of three genes in control of this trait.

KW - Cytokinin

KW - Explant sensitivity

KW - In vitro differentiation

KW - Inbred lines

KW - Phytohormone variation

UR - http://www.scopus.com/inward/record.url?scp=0031462076&partnerID=8YFLogxK

U2 - 10.1007/s11627-997-0048-0

DO - 10.1007/s11627-997-0048-0

M3 - Article

AN - SCOPUS:0031462076

VL - 33

SP - 269

EP - 274

JO - In Vitro

JF - In Vitro

SN - 0073-5655

IS - 4

ER -

ID: 93797877