TY - JOUR

T1 - Functional Analysis of TAAR1 Expression in the Intestine Wall and the Effect of Its Gene Knockout on the Gut Microbiota in Mice

AU - Ваганова, Анастасия Николаевна

AU - Жуков, Илья Сергеевич

AU - Шемякова, Таисия Сергеевна

AU - Рожков, Константин Александрович

AU - Алферова, Любовь Сергеевна

AU - Карасёва, Алёна

AU - Ермоленко, Елена Игоревна

AU - Гайнетдинов, Рауль Радикович

PY - 2024/12/9

Y1 - 2024/12/9

N2 - Currently, the TAAR1 receptor has been identified in various cell groups in the intestinal wall. It recognizes biogenic amine compounds like phenylethylamine or tyramine, which are products of decarboxylation of phenylalanine and tyrosine by endogenous or bacterial decarboxylases. Since several gut bacteria produce these amines, TAAR1 is suggested to be involved in the interaction between the host and gut microbiota. The purpose of this present study was to clarify the TAAR1 function in the intestinal wall and estimate the TAAR1 gene knockout effect on gut microbiota composition. By analyzing public transcriptomic data of the GEO repository, we identified TAAR1 expression in enterocytes, enteroendocrine cells, tuft cells, and myenteric neurons in mice. The analysis of genes co-expressed with TAAR1 in enteroendocrine cells allows us to suggest the TAAR1 involvement in enteroendocrine cell maturation. Also, in myenteric neurons, we identified the co-expression of TAAR1 with calbindin, which is specific for sensory neurons. The 16S rRNA gene-based analysis of fecal microbiota revealed a slight but significant impact of TAAR1 gene knockout in mice on the gut microbial community, which manifests in the higher diversity, accompanied by low between-sample variability and reorganization of the microbial co-occurrence network.

AB - Currently, the TAAR1 receptor has been identified in various cell groups in the intestinal wall. It recognizes biogenic amine compounds like phenylethylamine or tyramine, which are products of decarboxylation of phenylalanine and tyrosine by endogenous or bacterial decarboxylases. Since several gut bacteria produce these amines, TAAR1 is suggested to be involved in the interaction between the host and gut microbiota. The purpose of this present study was to clarify the TAAR1 function in the intestinal wall and estimate the TAAR1 gene knockout effect on gut microbiota composition. By analyzing public transcriptomic data of the GEO repository, we identified TAAR1 expression in enterocytes, enteroendocrine cells, tuft cells, and myenteric neurons in mice. The analysis of genes co-expressed with TAAR1 in enteroendocrine cells allows us to suggest the TAAR1 involvement in enteroendocrine cell maturation. Also, in myenteric neurons, we identified the co-expression of TAAR1 with calbindin, which is specific for sensory neurons. The 16S rRNA gene-based analysis of fecal microbiota revealed a slight but significant impact of TAAR1 gene knockout in mice on the gut microbial community, which manifests in the higher diversity, accompanied by low between-sample variability and reorganization of the microbial co-occurrence network.

KW - Animals

KW - Enteroendocrine Cells/metabolism

KW - Gastrointestinal Microbiome

KW - Intestinal Mucosa/metabolism

KW - Intestines/microbiology

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - RNA, Ribosomal, 16S/genetics

KW - Receptors, G-Protein-Coupled/genetics

KW - Transcriptome

KW - intestine

KW - TAAR1

KW - gut microbiota

KW - transcriptomic data

KW - enteroendocrine cells

KW - trace amine-associated receptor

KW - colon

KW - TAAR

KW - myenteric neurons

UR - https://www.mendeley.com/catalogue/7973cb00-5031-312b-8b7e-af29bb91e5c0/

U2 - 10.3390/ijms252313216

DO - 10.3390/ijms252313216

M3 - Article

C2 - 39684925

VL - 25

JO - International Journal of Medical Sciences

JF - International Journal of Medical Sciences

SN - 1449-1907

IS - 23

M1 - 13216

ER -