Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Functional Analysis of TAAR1 Expression in the Intestine Wall and the Effect of Its Gene Knockout on the Gut Microbiota in Mice. / Ваганова, Анастасия Николаевна; Жуков, Илья Сергеевич; Шемякова, Таисия Сергеевна; Рожков, Константин Александрович; Алферова, Любовь Сергеевна; Карасёва, Алёна; Ермоленко, Елена Игоревна; Гайнетдинов, Рауль Радикович.
в: International Journal of Medical Sciences, Том 25, № 23, 13216, 09.12.2024.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Functional Analysis of TAAR1 Expression in the Intestine Wall and the Effect of Its Gene Knockout on the Gut Microbiota in Mice
AU - Ваганова, Анастасия Николаевна
AU - Жуков, Илья Сергеевич
AU - Шемякова, Таисия Сергеевна
AU - Рожков, Константин Александрович
AU - Алферова, Любовь Сергеевна
AU - Карасёва, Алёна
AU - Ермоленко, Елена Игоревна
AU - Гайнетдинов, Рауль Радикович
PY - 2024/12/9
Y1 - 2024/12/9
N2 - Currently, the TAAR1 receptor has been identified in various cell groups in the intestinal wall. It recognizes biogenic amine compounds like phenylethylamine or tyramine, which are products of decarboxylation of phenylalanine and tyrosine by endogenous or bacterial decarboxylases. Since several gut bacteria produce these amines, TAAR1 is suggested to be involved in the interaction between the host and gut microbiota. The purpose of this present study was to clarify the TAAR1 function in the intestinal wall and estimate the TAAR1 gene knockout effect on gut microbiota composition. By analyzing public transcriptomic data of the GEO repository, we identified TAAR1 expression in enterocytes, enteroendocrine cells, tuft cells, and myenteric neurons in mice. The analysis of genes co-expressed with TAAR1 in enteroendocrine cells allows us to suggest the TAAR1 involvement in enteroendocrine cell maturation. Also, in myenteric neurons, we identified the co-expression of TAAR1 with calbindin, which is specific for sensory neurons. The 16S rRNA gene-based analysis of fecal microbiota revealed a slight but significant impact of TAAR1 gene knockout in mice on the gut microbial community, which manifests in the higher diversity, accompanied by low between-sample variability and reorganization of the microbial co-occurrence network.
AB - Currently, the TAAR1 receptor has been identified in various cell groups in the intestinal wall. It recognizes biogenic amine compounds like phenylethylamine or tyramine, which are products of decarboxylation of phenylalanine and tyrosine by endogenous or bacterial decarboxylases. Since several gut bacteria produce these amines, TAAR1 is suggested to be involved in the interaction between the host and gut microbiota. The purpose of this present study was to clarify the TAAR1 function in the intestinal wall and estimate the TAAR1 gene knockout effect on gut microbiota composition. By analyzing public transcriptomic data of the GEO repository, we identified TAAR1 expression in enterocytes, enteroendocrine cells, tuft cells, and myenteric neurons in mice. The analysis of genes co-expressed with TAAR1 in enteroendocrine cells allows us to suggest the TAAR1 involvement in enteroendocrine cell maturation. Also, in myenteric neurons, we identified the co-expression of TAAR1 with calbindin, which is specific for sensory neurons. The 16S rRNA gene-based analysis of fecal microbiota revealed a slight but significant impact of TAAR1 gene knockout in mice on the gut microbial community, which manifests in the higher diversity, accompanied by low between-sample variability and reorganization of the microbial co-occurrence network.
KW - Animals
KW - Enteroendocrine Cells/metabolism
KW - Gastrointestinal Microbiome
KW - Intestinal Mucosa/metabolism
KW - Intestines/microbiology
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - RNA, Ribosomal, 16S/genetics
KW - Receptors, G-Protein-Coupled/genetics
KW - Transcriptome
KW - intestine
KW - TAAR1
KW - gut microbiota
KW - transcriptomic data
KW - enteroendocrine cells
KW - trace amine-associated receptor
KW - colon
KW - TAAR
KW - myenteric neurons
UR - https://www.mendeley.com/catalogue/7973cb00-5031-312b-8b7e-af29bb91e5c0/
U2 - 10.3390/ijms252313216
DO - 10.3390/ijms252313216
M3 - Article
C2 - 39684925
VL - 25
JO - International Journal of Medical Sciences
JF - International Journal of Medical Sciences
SN - 1449-1907
IS - 23
M1 - 13216
ER -
ID: 128234915