TY - JOUR

T1 - Fluoroquinolones extraction from meat samples based on deep eutectic solvent formation

AU - Timofeeva, Irina

AU - Stepanova, Kira

AU - Shishov, Andrey

AU - Nugbienyo, Lawrence

AU - Moskvin, Leonid

AU - Bulatov, Andrey

PY - 2020/10

Y1 - 2020/10

N2 - A simple, highly available and cheap approach for effective pretreatment of meat samples, based on the formation of deep eutectic solvent (DES), was proposed for the first time. The procedure assumed dissolution of a hydrogen bond acceptor of DES (quaternary ammonium salt) in homogenized meat sample, followed by heating with a hydrogen bond donor of DES (medium-chain alcohol). Heating the mixture promoted DES formation and separation of analytes from the meat sample into the DES phase formed. The developed approach was proven to be highly effective for ofloxacin and fleroxacin separation from meat samples (chicken and beef). It was shown, that the developed procedure provided higher extraction efficiency of analytes (extraction recovery 98–100 %) in comparison with conventional extraction in pre-synthesized viscous DES, composed of tetrabutylammonium bromide and heptanol (extraction recovery 33–67 %). The developed approach was coupled with high-performance liquid chromatography with fluorometric detection for the determination of ofloxacin and fleroxacin in real and spiked meat samples. The limits of detection obtained from peaks 3 times the signal-to-noise ratio were 10 μg kg−1 for ofloxacin and 15 μg kg−1 for fleroxacin.

AB - A simple, highly available and cheap approach for effective pretreatment of meat samples, based on the formation of deep eutectic solvent (DES), was proposed for the first time. The procedure assumed dissolution of a hydrogen bond acceptor of DES (quaternary ammonium salt) in homogenized meat sample, followed by heating with a hydrogen bond donor of DES (medium-chain alcohol). Heating the mixture promoted DES formation and separation of analytes from the meat sample into the DES phase formed. The developed approach was proven to be highly effective for ofloxacin and fleroxacin separation from meat samples (chicken and beef). It was shown, that the developed procedure provided higher extraction efficiency of analytes (extraction recovery 98–100 %) in comparison with conventional extraction in pre-synthesized viscous DES, composed of tetrabutylammonium bromide and heptanol (extraction recovery 33–67 %). The developed approach was coupled with high-performance liquid chromatography with fluorometric detection for the determination of ofloxacin and fleroxacin in real and spiked meat samples. The limits of detection obtained from peaks 3 times the signal-to-noise ratio were 10 μg kg−1 for ofloxacin and 15 μg kg−1 for fleroxacin.

KW - Fluoroquinolones

KW - HHPLC-FLD

KW - Meat

KW - Medium-Chain alcohol

KW - Quaternary ammonium salt

KW - ACID

KW - CHOLINE CHLORIDE

KW - PESTICIDES

KW - LIQUID-LIQUID MICROEXTRACTION

KW - FISH

KW - IMMUNOASSAY

KW - RESIDUES

KW - DETERMINING CU

KW - WATER

KW - FOOD

UR - http://www.scopus.com/inward/record.url?scp=85088153925&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/af8fd57d-9ed9-376c-b0ab-436636d9c157/

U2 - 10.1016/j.jfca.2020.103589

DO - 10.1016/j.jfca.2020.103589

M3 - Article

AN - SCOPUS:85088153925

VL - 93

JO - Journal of Food Composition and Analysis

JF - Journal of Food Composition and Analysis

SN - 0889-1575

M1 - 103589

ER -