Sequencing of rye genome in 2021 gave research community a great opportunity for identifying specific genes underlying previously described mutations. Our interest is in flavonoid biosynthesis in rye grain. Previously, six mutations causing absence of anthocyanins color in grain and other organs and also different flavonoid content in rye grains were identified. Based on genomic sequences of two published rye genomes a new annotation and search for genes involved in flavonoid synthesis were performed. To confirm functionality of the genes trancriptomic data were also used in research. Resulting map of flavonoid synthesis genes on rye chromosomes is a powerful tool for identifying candidate genes for anthocyaninless mutations.
The work was supported by the Ministry of Science and Higher Education of the Russian Federation in accordance with agreement No. 075-15-2022-322 date 22.04.2022 on providing a grant in the form of subsidies from the Federal budget of Russian Federation.