TY - JOUR

T1 - Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae

AU - Rumyantsev, A.M.

AU - Zakharov, G.A.

AU - Zhuravlev, A.V.

AU - Padkina, M.V.

AU - Savvateeva-Popova, E.V.

AU - Sambuk, E.V.

PY - 2014

Y1 - 2014

N2 - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U

AB - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U

UR - http://www.scopus.com/inward/record.url?scp=84938520452&partnerID=8YFLogxK

U2 - 10.1134/S102279541406009X

DO - 10.1134/S102279541406009X

M3 - Article

C2 - 25715455

VL - 50

SP - 569

EP - 576

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 6

ER -