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Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae. / Rumyantsev, A.M.; Zakharov, G.A.; Zhuravlev, A.V.; Padkina, M.V.; Savvateeva-Popova, E.V.; Sambuk, E.V.

In: Russian Journal of Genetics, Vol. 50, No. 6, 2014, p. 569-576.

Research output: Contribution to journal › Article › peer-review

Harvard

Rumyantsev, AM, Zakharov, GA, Zhuravlev, AV, Padkina, MV, Savvateeva-Popova, EV & Sambuk, EV 2014, 'Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae', Russian Journal of Genetics, vol. 50, no. 6, pp. 569-576. https://doi.org/10.1134/S102279541406009X

APA

Rumyantsev, A. M., Zakharov, G. A., Zhuravlev, A. V., Padkina, M. V., Savvateeva-Popova, E. V., & Sambuk, E. V. (2014). Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae. Russian Journal of Genetics, 50(6), 569-576. https://doi.org/10.1134/S102279541406009X

Vancouver

Rumyantsev AM, Zakharov GA, Zhuravlev AV, Padkina MV, Savvateeva-Popova EV, Sambuk EV. Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae. Russian Journal of Genetics. 2014;50(6):569-576. https://doi.org/10.1134/S102279541406009X

Author

Rumyantsev, A.M. ; Zakharov, G.A. ; Zhuravlev, A.V. ; Padkina, M.V. ; Savvateeva-Popova, E.V. ; Sambuk, E.V. / Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae. In: Russian Journal of Genetics. 2014 ; Vol. 50, No. 6. pp. 569-576.

BibTeX

@article{964b39eceafe4f8585beb1e9698cd16d,
title = "Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae",
abstract = "The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U",
author = "A.M. Rumyantsev and G.A. Zakharov and A.V. Zhuravlev and M.V. Padkina and E.V. Savvateeva-Popova and E.V. Sambuk",
year = "2014",
doi = "10.1134/S102279541406009X",
language = "English",
volume = "50",
pages = "569--576",
journal = "Russian Journal of Genetics",
issn = "1022-7954",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "6",

}

RIS

TY - JOUR

T1 - Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae

AU - Rumyantsev, A.M.

AU - Zakharov, G.A.

AU - Zhuravlev, A.V.

AU - Padkina, M.V.

AU - Savvateeva-Popova, E.V.

AU - Sambuk, E.V.

PY - 2014

Y1 - 2014

N2 - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U

AB - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U

UR - http://www.scopus.com/inward/record.url?scp=84938520452&partnerID=8YFLogxK

U2 - 10.1134/S102279541406009X

DO - 10.1134/S102279541406009X

M3 - Article

C2 - 25715455

VL - 50

SP - 569

EP - 576

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 6

ER -

ID: 7036764