TY - JOUR

T1 - Effective delivery of porphyrazine photosensitizers to cancer cells by polymer brush nanocontainers

AU - Shilyagina, Natalia Y.

AU - Peskova, Nina N.

AU - Lermontova, Svetlana A.

AU - Brilkina, Anna A.

AU - Vodeneev, Vladimir A.

AU - Yakimansky, Alexander V.

AU - Klapshina, Larisa G.

AU - Balalaeva, Irina V.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Efficient drug delivery can be assigned to tasks that attract the most acute attention of researchers in the field of anticancer drug design. We have reported the first case of using amphiphilic polymer brushes as nanocontainers for photosensitizer delivery to cancer cells. Regular graft-copolymers of hydrophobic polyimides with hydrophilic polymethacrylic acid side chains were loaded with photosensitive dye tetra(4-fluorophenyl)tetracyanoporphyrazine (Pz) providing a sufficiently stable homogeneous fraction of fluorescent Pz-loaded nanoparticles with a size of 100–150 nm. Pz-loaded polymer brushes were substantially more efficient for Pz delivery into cells compared with other types of particles examined, Pz-polyethyleneglycol and Pz-methylcellulose. In vivo, an efficient Pz delivery to tumor can also be expected since the Pz-PB particle size is in the optimal range for passive targeting. Pz-PB showed pronounced photodynamic activity, while, that is important, in the absence of irradiation the PB carrier itself was significantly less toxic than the dye itself. (Figure presented.) Summing up, water-soluble polymer brushes with polyimide backbones and polymethacrylic acid side chains can be regarded as a novel type of nanocontainers providing efficient intracellular drug delivery for photodynamic therapy of cancers.

AB - Efficient drug delivery can be assigned to tasks that attract the most acute attention of researchers in the field of anticancer drug design. We have reported the first case of using amphiphilic polymer brushes as nanocontainers for photosensitizer delivery to cancer cells. Regular graft-copolymers of hydrophobic polyimides with hydrophilic polymethacrylic acid side chains were loaded with photosensitive dye tetra(4-fluorophenyl)tetracyanoporphyrazine (Pz) providing a sufficiently stable homogeneous fraction of fluorescent Pz-loaded nanoparticles with a size of 100–150 nm. Pz-loaded polymer brushes were substantially more efficient for Pz delivery into cells compared with other types of particles examined, Pz-polyethyleneglycol and Pz-methylcellulose. In vivo, an efficient Pz delivery to tumor can also be expected since the Pz-PB particle size is in the optimal range for passive targeting. Pz-PB showed pronounced photodynamic activity, while, that is important, in the absence of irradiation the PB carrier itself was significantly less toxic than the dye itself. (Figure presented.) Summing up, water-soluble polymer brushes with polyimide backbones and polymethacrylic acid side chains can be regarded as a novel type of nanocontainers providing efficient intracellular drug delivery for photodynamic therapy of cancers.

KW - drug delivery

KW - fluorescent molecular rotors

KW - photosensitizers

KW - polymer brushes

KW - porphyrazines

UR - http://www.scopus.com/inward/record.url?scp=85006086315&partnerID=8YFLogxK

U2 - 10.1002/jbio.201600212

DO - 10.1002/jbio.201600212

M3 - Article

AN - SCOPUS:85006086315

VL - 10

SP - 1189

EP - 1197

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 9

ER -