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Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress. / Soboleva, Alena; Frolova, Nadezhda; Bureiko, Kseniia; Shumilina, Julia; Balcke, Gerd U.; Zhukov, Vladimir A.; Tikhonovich, Igor A.; Frolov, Andrej.

в: International Journal of Molecular Sciences, Том 23, № 5, 2726, 01.03.2022.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

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@article{db155046a69c4938b4f26d124fabd587,
title = "Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress",
abstract = "Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultimately accompanies plants{\textquoteright} response to water deficit. Indeed, enhanced formation of reactive oxygen species in root nodules might result in up-regulation of lipid peroxidation and overproduction of reactive carbonyl compounds (RCCs), which readily modify biomolecules and disrupt cell functions. Thus, the knowledge of the nodule carbonyl metabolome dynamics is critically important for understanding the drought-related losses of nitrogen fixation efficiency and plant productivity. Therefore, here we provide, to the best of our knowledge, for the first time a comprehensive overview of the pea root nodule carbonyl metabolome and address its alterations in response to polyethylene glycol-induced osmotic stress as the first step to examine the changes of RCC patterns in drought treated plants. RCCs were extracted from the nodules and derivatized with 7-(diethylamino)coumarin-3-carbohydrazide (CHH). The relative quantification of CHH-derivatives by liquid chromatography-high resolution mass spectrometry with a post-run correction for derivative stability revealed in total 194 features with intensities above 1 × 105 counts, 19 of which were down-and three were upregulated. The upregulation of glyceraldehyde could accompany non-enzymatic conversion of glyceraldehyde-3-phosphate to methylglyoxal. The accumulation of 4,5-dioxovaleric acid could be the reason for down-regulation of porphyrin metabolism, suppression of leghemoglobin synthesis, inhibition of nitrogenase and degradation of legume-rhizobial symbiosis in response to polyethylene glycol (PEG)-induced osmotic stress effect. This effect needs to be confirmed with soil-based drought models.",
keywords = "4,5-dioxovaleric acid, 7-(diethylamino)coumarin-3-carbohydrazide (CHH), Derivative stability, Drought, Legume-rhizobial symbiosis, Metabolomics, Osmotic stress, Pea (Pisum sativum L.), Reactive carbonyl compounds (RCCs), Root nodules, Symbiosis, Osmotic Pressure, Polyethylene Glycols/metabolism, Peas/metabolism, Glyceraldehyde, Fabaceae/metabolism, Root Nodules, Plant/metabolism, Rhizobium/metabolism, Nitrogen Fixation, 5-dioxovaleric acid, 4,5-DIOXOVALERIC ACID, PHYSIOLOGICAL-PARAMETERS, DROUGHT STRESS, PROTEIN CARBONYLATION, pea (Pisum sativum L, QUALITY-CONTROL, legume-rhizobial symbiosis, PISUM-SATIVUM, reactive carbonyl compounds (RCCs), osmotic stress, metabolomics, GENE-EXPRESSION, HYDROGEN-PEROXIDE, root nodules, OXIDATIVE STRESS, derivative stability, LIPID-PEROXIDATION, ), 4, drought",
author = "Alena Soboleva and Nadezhda Frolova and Kseniia Bureiko and Julia Shumilina and Balcke, {Gerd U.} and Zhukov, {Vladimir A.} and Tikhonovich, {Igor A.} and Andrej Frolov",
note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = mar,
day = "1",
doi = "10.3390/ijms23052726",
language = "English",
volume = "23",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI AG",
number = "5",

}

RIS

TY - JOUR

T1 - Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

AU - Soboleva, Alena

AU - Frolova, Nadezhda

AU - Bureiko, Kseniia

AU - Shumilina, Julia

AU - Balcke, Gerd U.

AU - Zhukov, Vladimir A.

AU - Tikhonovich, Igor A.

AU - Frolov, Andrej

N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/3/1

Y1 - 2022/3/1

N2 - Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultimately accompanies plants’ response to water deficit. Indeed, enhanced formation of reactive oxygen species in root nodules might result in up-regulation of lipid peroxidation and overproduction of reactive carbonyl compounds (RCCs), which readily modify biomolecules and disrupt cell functions. Thus, the knowledge of the nodule carbonyl metabolome dynamics is critically important for understanding the drought-related losses of nitrogen fixation efficiency and plant productivity. Therefore, here we provide, to the best of our knowledge, for the first time a comprehensive overview of the pea root nodule carbonyl metabolome and address its alterations in response to polyethylene glycol-induced osmotic stress as the first step to examine the changes of RCC patterns in drought treated plants. RCCs were extracted from the nodules and derivatized with 7-(diethylamino)coumarin-3-carbohydrazide (CHH). The relative quantification of CHH-derivatives by liquid chromatography-high resolution mass spectrometry with a post-run correction for derivative stability revealed in total 194 features with intensities above 1 × 105 counts, 19 of which were down-and three were upregulated. The upregulation of glyceraldehyde could accompany non-enzymatic conversion of glyceraldehyde-3-phosphate to methylglyoxal. The accumulation of 4,5-dioxovaleric acid could be the reason for down-regulation of porphyrin metabolism, suppression of leghemoglobin synthesis, inhibition of nitrogenase and degradation of legume-rhizobial symbiosis in response to polyethylene glycol (PEG)-induced osmotic stress effect. This effect needs to be confirmed with soil-based drought models.

AB - Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultimately accompanies plants’ response to water deficit. Indeed, enhanced formation of reactive oxygen species in root nodules might result in up-regulation of lipid peroxidation and overproduction of reactive carbonyl compounds (RCCs), which readily modify biomolecules and disrupt cell functions. Thus, the knowledge of the nodule carbonyl metabolome dynamics is critically important for understanding the drought-related losses of nitrogen fixation efficiency and plant productivity. Therefore, here we provide, to the best of our knowledge, for the first time a comprehensive overview of the pea root nodule carbonyl metabolome and address its alterations in response to polyethylene glycol-induced osmotic stress as the first step to examine the changes of RCC patterns in drought treated plants. RCCs were extracted from the nodules and derivatized with 7-(diethylamino)coumarin-3-carbohydrazide (CHH). The relative quantification of CHH-derivatives by liquid chromatography-high resolution mass spectrometry with a post-run correction for derivative stability revealed in total 194 features with intensities above 1 × 105 counts, 19 of which were down-and three were upregulated. The upregulation of glyceraldehyde could accompany non-enzymatic conversion of glyceraldehyde-3-phosphate to methylglyoxal. The accumulation of 4,5-dioxovaleric acid could be the reason for down-regulation of porphyrin metabolism, suppression of leghemoglobin synthesis, inhibition of nitrogenase and degradation of legume-rhizobial symbiosis in response to polyethylene glycol (PEG)-induced osmotic stress effect. This effect needs to be confirmed with soil-based drought models.

KW - 4,5-dioxovaleric acid

KW - 7-(diethylamino)coumarin-3-carbohydrazide (CHH)

KW - Derivative stability

KW - Drought

KW - Legume-rhizobial symbiosis

KW - Metabolomics

KW - Osmotic stress

KW - Pea (Pisum sativum L.)

KW - Reactive carbonyl compounds (RCCs)

KW - Root nodules

KW - Symbiosis

KW - Osmotic Pressure

KW - Polyethylene Glycols/metabolism

KW - Peas/metabolism

KW - Glyceraldehyde

KW - Fabaceae/metabolism

KW - Root Nodules, Plant/metabolism

KW - Rhizobium/metabolism

KW - Nitrogen Fixation

KW - 5-dioxovaleric acid

KW - 4,5-DIOXOVALERIC ACID

KW - PHYSIOLOGICAL-PARAMETERS

KW - DROUGHT STRESS

KW - PROTEIN CARBONYLATION

KW - pea (Pisum sativum L

KW - QUALITY-CONTROL

KW - legume-rhizobial symbiosis

KW - PISUM-SATIVUM

KW - reactive carbonyl compounds (RCCs)

KW - osmotic stress

KW - metabolomics

KW - GENE-EXPRESSION

KW - HYDROGEN-PEROXIDE

KW - root nodules

KW - OXIDATIVE STRESS

KW - derivative stability

KW - LIPID-PEROXIDATION

KW - )

KW - 4

KW - drought

UR - http://www.scopus.com/inward/record.url?scp=85125426173&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/9e08ac56-ea1a-37fa-b417-0bae5c9cd431/

U2 - 10.3390/ijms23052726

DO - 10.3390/ijms23052726

M3 - Article

C2 - 35269869

AN - SCOPUS:85125426173

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 5

M1 - 2726

ER -

ID: 93358869