Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress. / Soboleva, Alena; Frolova, Nadezhda; Bureiko, Kseniia; Shumilina, Julia; Balcke, Gerd U.; Zhukov, Vladimir A.; Tikhonovich, Igor A.; Frolov, Andrej.
в: International Journal of Molecular Sciences, Том 23, № 5, 2726, 01.03.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress
AU - Soboleva, Alena
AU - Frolova, Nadezhda
AU - Bureiko, Kseniia
AU - Shumilina, Julia
AU - Balcke, Gerd U.
AU - Zhukov, Vladimir A.
AU - Tikhonovich, Igor A.
AU - Frolov, Andrej
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultimately accompanies plants’ response to water deficit. Indeed, enhanced formation of reactive oxygen species in root nodules might result in up-regulation of lipid peroxidation and overproduction of reactive carbonyl compounds (RCCs), which readily modify biomolecules and disrupt cell functions. Thus, the knowledge of the nodule carbonyl metabolome dynamics is critically important for understanding the drought-related losses of nitrogen fixation efficiency and plant productivity. Therefore, here we provide, to the best of our knowledge, for the first time a comprehensive overview of the pea root nodule carbonyl metabolome and address its alterations in response to polyethylene glycol-induced osmotic stress as the first step to examine the changes of RCC patterns in drought treated plants. RCCs were extracted from the nodules and derivatized with 7-(diethylamino)coumarin-3-carbohydrazide (CHH). The relative quantification of CHH-derivatives by liquid chromatography-high resolution mass spectrometry with a post-run correction for derivative stability revealed in total 194 features with intensities above 1 × 105 counts, 19 of which were down-and three were upregulated. The upregulation of glyceraldehyde could accompany non-enzymatic conversion of glyceraldehyde-3-phosphate to methylglyoxal. The accumulation of 4,5-dioxovaleric acid could be the reason for down-regulation of porphyrin metabolism, suppression of leghemoglobin synthesis, inhibition of nitrogenase and degradation of legume-rhizobial symbiosis in response to polyethylene glycol (PEG)-induced osmotic stress effect. This effect needs to be confirmed with soil-based drought models.
AB - Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultimately accompanies plants’ response to water deficit. Indeed, enhanced formation of reactive oxygen species in root nodules might result in up-regulation of lipid peroxidation and overproduction of reactive carbonyl compounds (RCCs), which readily modify biomolecules and disrupt cell functions. Thus, the knowledge of the nodule carbonyl metabolome dynamics is critically important for understanding the drought-related losses of nitrogen fixation efficiency and plant productivity. Therefore, here we provide, to the best of our knowledge, for the first time a comprehensive overview of the pea root nodule carbonyl metabolome and address its alterations in response to polyethylene glycol-induced osmotic stress as the first step to examine the changes of RCC patterns in drought treated plants. RCCs were extracted from the nodules and derivatized with 7-(diethylamino)coumarin-3-carbohydrazide (CHH). The relative quantification of CHH-derivatives by liquid chromatography-high resolution mass spectrometry with a post-run correction for derivative stability revealed in total 194 features with intensities above 1 × 105 counts, 19 of which were down-and three were upregulated. The upregulation of glyceraldehyde could accompany non-enzymatic conversion of glyceraldehyde-3-phosphate to methylglyoxal. The accumulation of 4,5-dioxovaleric acid could be the reason for down-regulation of porphyrin metabolism, suppression of leghemoglobin synthesis, inhibition of nitrogenase and degradation of legume-rhizobial symbiosis in response to polyethylene glycol (PEG)-induced osmotic stress effect. This effect needs to be confirmed with soil-based drought models.
KW - 4,5-dioxovaleric acid
KW - 7-(diethylamino)coumarin-3-carbohydrazide (CHH)
KW - Derivative stability
KW - Drought
KW - Legume-rhizobial symbiosis
KW - Metabolomics
KW - Osmotic stress
KW - Pea (Pisum sativum L.)
KW - Reactive carbonyl compounds (RCCs)
KW - Root nodules
KW - Symbiosis
KW - Osmotic Pressure
KW - Polyethylene Glycols/metabolism
KW - Peas/metabolism
KW - Glyceraldehyde
KW - Fabaceae/metabolism
KW - Root Nodules, Plant/metabolism
KW - Rhizobium/metabolism
KW - Nitrogen Fixation
KW - 5-dioxovaleric acid
KW - 4,5-DIOXOVALERIC ACID
KW - PHYSIOLOGICAL-PARAMETERS
KW - DROUGHT STRESS
KW - PROTEIN CARBONYLATION
KW - pea (Pisum sativum L
KW - QUALITY-CONTROL
KW - legume-rhizobial symbiosis
KW - PISUM-SATIVUM
KW - reactive carbonyl compounds (RCCs)
KW - osmotic stress
KW - metabolomics
KW - GENE-EXPRESSION
KW - HYDROGEN-PEROXIDE
KW - root nodules
KW - OXIDATIVE STRESS
KW - derivative stability
KW - LIPID-PEROXIDATION
KW - )
KW - 4
KW - drought
UR - http://www.scopus.com/inward/record.url?scp=85125426173&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/9e08ac56-ea1a-37fa-b417-0bae5c9cd431/
U2 - 10.3390/ijms23052726
DO - 10.3390/ijms23052726
M3 - Article
C2 - 35269869
AN - SCOPUS:85125426173
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 5
M1 - 2726
ER -
ID: 93358869