TY - JOUR

T1 - Dynamics of Hydrogen Peroxide Accumulation During Tip Growth of Infection Thread in Nodules and Cell Differentiation in Pea (Pisum sativum L.) Symbiotic Nodules

AU - Tsyganova, A.V.

AU - Gorshkov, A.P.

AU - Vorobiev, M.G.

AU - Tikhonovich, I.A.

AU - Brewin, N.J.

AU - Tsyganov, V.E.

N1 - Export Date: 10 November 2024 Сведения о финансировании: Russian Science Foundation, RSF, 23-16-00090 Сведения о финансировании: Russian Science Foundation, RSF Текст о финансировании 1: This research was funded by the Russian Science Foundation, grant number 23-16-00090.

PY - 2024/10/18

Y1 - 2024/10/18

N2 - Hydrogen peroxide (H2O2) in plants is produced in relatively large amounts and plays a universal role in plant defense and physiological responses, including the regulation of growth and development. In the Rhizobium–legume symbiosis, hydrogen peroxide plays an important signaling role throughout the development of this interaction. In the functioning nodule, H2O2 has been shown to be involved in bacterial differentiation into the symbiotic form and in nodule senescence. In this study, the pattern of H2O2 accumulation in pea (Pisum sativum L.) wild-type and mutant nodules blocked at different stages of the infection process was analyzed using a cytochemical reaction with cerium chloride. The observed dynamics of H2O2 deposition in the infection thread walls indicated that the distribution of H2O2 was apparently related to the stiffness of the infection thread wall. The dynamics of H2O2 accumulation was traced, and its patterns in different nodule zones were determined in order to investigate the relationship of H2O2 localization and distribution with the stages of symbiotic nodule development in P. sativum. The patterns of H2O2 localization in different zones of the indeterminate nodule have been partially confirmed by comparative analysis on mutant genotypes. © 2024 by the authors.

AB - Hydrogen peroxide (H2O2) in plants is produced in relatively large amounts and plays a universal role in plant defense and physiological responses, including the regulation of growth and development. In the Rhizobium–legume symbiosis, hydrogen peroxide plays an important signaling role throughout the development of this interaction. In the functioning nodule, H2O2 has been shown to be involved in bacterial differentiation into the symbiotic form and in nodule senescence. In this study, the pattern of H2O2 accumulation in pea (Pisum sativum L.) wild-type and mutant nodules blocked at different stages of the infection process was analyzed using a cytochemical reaction with cerium chloride. The observed dynamics of H2O2 deposition in the infection thread walls indicated that the distribution of H2O2 was apparently related to the stiffness of the infection thread wall. The dynamics of H2O2 accumulation was traced, and its patterns in different nodule zones were determined in order to investigate the relationship of H2O2 localization and distribution with the stages of symbiotic nodule development in P. sativum. The patterns of H2O2 localization in different zones of the indeterminate nodule have been partially confirmed by comparative analysis on mutant genotypes. © 2024 by the authors.

KW - hydrogen peroxide

KW - infection thread

KW - Pisum sativumL

KW - polar growth

KW - Rhizobium–legume symbiosis

KW - root nodule

UR - https://www.mendeley.com/catalogue/c4305350-df53-3dcd-a108-63a549fb643e/

U2 - 10.3390/plants13202923

DO - 10.3390/plants13202923

M3 - статья

C2 - 39458872

VL - 13

JO - Plants

JF - Plants

SN - 2223-7747

IS - 20

M1 - 2923

ER -