Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Diversity of arbuscular mycorrhizal fungi in distinct ecosystems of the North Caucasus, a temperate biodiversity hotspot. / Юрков, Андрей Павлович; Крюков, А.А.; Горбунова, А.О.; Кудряшова, Т.Р.; Ковальчук, А.И.; Горенкова, А.И.; Богданова, Екатерина Михайловна; Лактионов, Юрий В.; Журбенко, Петр Михайлович; Михайлова, Юлия; Пузанский, Роман Константинович; Багрова, Татьяна Николаевна; Яхин, О.И.; Родионов, Александр Викентьевич; Шишова, Мария Федоровна.
в: Journal of Fungi, Том 10, № 1, 11, 2024.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Diversity of arbuscular mycorrhizal fungi in distinct ecosystems of the North Caucasus, a temperate biodiversity hotspot
AU - Юрков, Андрей Павлович
AU - Крюков, А.А.
AU - Горбунова, А.О.
AU - Кудряшова, Т.Р.
AU - Ковальчук, А.И.
AU - Горенкова, А.И.
AU - Богданова, Екатерина Михайловна
AU - Лактионов, Юрий В.
AU - Журбенко, Петр Михайлович
AU - Михайлова, Юлия
AU - Пузанский, Роман Константинович
AU - Багрова, Татьяна Николаевна
AU - Яхин, О.И.
AU - Родионов, Александр Викентьевич
AU - Шишова, Мария Федоровна
N1 - Yurkov A.P., Kryukov A.A., Gorbunova A.O., Kudriashova T.R., Kovalchuk A.I., Gorenkova A.I., Bogdanova E.M., Laktionov Y.V., Zhurbenko P.M., Mikhaylova Y.V., Puzanskiy R.K., Bagrova T.N., Yakhin O.I., Rodionov A.V., Shishova M.F. Diversity of arbuscular mycorrhizal fungi in distinct ecosystems of the North Caucasus, a temperate biodiversity hotspot // Journal of Fungi. 2024. Vol. 10, No. 1. 11. https://doi.org/10.3390/jof10010011
PY - 2024
Y1 - 2024
N2 - BACKGROUND: Investigations that are focused on arbuscular mycorrhizal fungus (AMF) biodiversity is still limited. The analysis of the AMF taxa in the North Caucasus, a temperate biodiversity hotspot, used to be limited to the genus level. This study aimed to define the AMF biodiversity at the species level in the North Caucasus biotopes. METHODS: The molecular genetic identification of fungi was carried out with ITS1 and ITS2 regions as barcodes via sequencing using Illumina MiSeq, the analysis of phylogenetic trees for individual genera, and searches for operational taxonomic units (OTUs) with identification at the species level. Sequences from MaarjAM and NCBI GenBank were used as references. RESULTS: We analyzed >10 million reads in soil samples for three biotopes to estimate fungal biodiversity. Briefly, 50 AMF species belonging to 20 genera were registered. The total number of the AM fungus OTUs for the "Subalpine Meadow" biotope was 171/131, that for "Forest" was 117/60, and that for "River Valley" was 296/221 based on ITS1/ITS2 data. The total number of the AM fungus species (except for virtual taxa) for the "Subalpine Meadow" biotope was 24/19, that for "Forest" was 22/13, and that for "River Valley" was 28/24 based on ITS1/ITS2 data. Greater AMF diversity, as well as number of OTUs and species, in comparison with that of forest biotopes, characterized valley biotopes (disturbed ecosystems; grasslands). The correlation coefficient between "Percentage of annual plants" and "Glomeromycota total reads" r = 0.76 and 0.81 for ITS1 and ITS2, respectively, and the correlation coefficient between "Percentage of annual plants" and "OTUs number (for total species)" was r = 0.67 and 0.77 for ITS1 and ITS2, respectively. CONCLUSION: High AMF biodiversity for the river valley can be associated with a higher percentage of annual plants in these biotopes and the active development of restorative successional processes.
AB - BACKGROUND: Investigations that are focused on arbuscular mycorrhizal fungus (AMF) biodiversity is still limited. The analysis of the AMF taxa in the North Caucasus, a temperate biodiversity hotspot, used to be limited to the genus level. This study aimed to define the AMF biodiversity at the species level in the North Caucasus biotopes. METHODS: The molecular genetic identification of fungi was carried out with ITS1 and ITS2 regions as barcodes via sequencing using Illumina MiSeq, the analysis of phylogenetic trees for individual genera, and searches for operational taxonomic units (OTUs) with identification at the species level. Sequences from MaarjAM and NCBI GenBank were used as references. RESULTS: We analyzed >10 million reads in soil samples for three biotopes to estimate fungal biodiversity. Briefly, 50 AMF species belonging to 20 genera were registered. The total number of the AM fungus OTUs for the "Subalpine Meadow" biotope was 171/131, that for "Forest" was 117/60, and that for "River Valley" was 296/221 based on ITS1/ITS2 data. The total number of the AM fungus species (except for virtual taxa) for the "Subalpine Meadow" biotope was 24/19, that for "Forest" was 22/13, and that for "River Valley" was 28/24 based on ITS1/ITS2 data. Greater AMF diversity, as well as number of OTUs and species, in comparison with that of forest biotopes, characterized valley biotopes (disturbed ecosystems; grasslands). The correlation coefficient between "Percentage of annual plants" and "Glomeromycota total reads" r = 0.76 and 0.81 for ITS1 and ITS2, respectively, and the correlation coefficient between "Percentage of annual plants" and "OTUs number (for total species)" was r = 0.67 and 0.77 for ITS1 and ITS2, respectively. CONCLUSION: High AMF biodiversity for the river valley can be associated with a higher percentage of annual plants in these biotopes and the active development of restorative successional processes.
KW - Биоразнообразие
KW - арбускулярно-микоризные грибы
KW - Glomeromycetes
KW - Кавказ
KW - ITS1
KW - ITS2
KW - горячая точка биоразнообразия
KW - Caucasus
KW - Glomeromycetes
KW - ITS1
KW - ITS2
KW - arbuscular mycorrhizal fungi
KW - biodiversity
KW - hotspot
UR - https://www.mendeley.com/catalogue/6911e8b0-8016-3182-bea5-6486a8d165eb/
U2 - 10.3390/jof10010011
DO - 10.3390/jof10010011
M3 - Article
C2 - 38248921
VL - 10
JO - Journal of Fungi
JF - Journal of Fungi
SN - 2309-608X
IS - 1
M1 - 11
ER -
ID: 119468451