TY - JOUR

T1 - Distinctive features of the human marginal zone and Cajal–Retzius cells: comparison of morphological and immunocytochemical features at midgestation

AU - Tkachenko, L.A.

AU - Zykin, P.A.

AU - Nasyrov, R.A.

AU - Krasnoshchekova, E.I.

PY - 2016

Y1 - 2016

N2 - Despite a long history of research of cortical marginal zone (MZ) organization and development, a number of issues remain unresolved. One particular issue is the problem of Cajal–Retzius cells (C–R) identification. It is currently based on morphology and Reelin expression. The aim of this research is to investigate MZ cytoarchitectonics and Reelin-producing cells morphotypes in the superior temporal, pre- and postcentral cortex at GW24–26. We used Reelin (Reln) as the marker for C–R cells and microtubule-associated protein 2 (MAP2) and neurofilament heavy chain protein (N200) as markers of neuronal maturation. The MZ of all of the investigated areas had the distinct cytoarchitectonic of alternating cell sparse (MZP, SR) and cell dense (SGL, DGL) layers. The distribution of the neuromarkers across the MZ also showed layer specificity. MAP2-positive cells were only found in the SGL. N200 and Reelin-positive neurons in the MZP. N200-positive processes were forming a plexus at the DGL level. All of the N200-posit

AB - Despite a long history of research of cortical marginal zone (MZ) organization and development, a number of issues remain unresolved. One particular issue is the problem of Cajal–Retzius cells (C–R) identification. It is currently based on morphology and Reelin expression. The aim of this research is to investigate MZ cytoarchitectonics and Reelin-producing cells morphotypes in the superior temporal, pre- and postcentral cortex at GW24–26. We used Reelin (Reln) as the marker for C–R cells and microtubule-associated protein 2 (MAP2) and neurofilament heavy chain protein (N200) as markers of neuronal maturation. The MZ of all of the investigated areas had the distinct cytoarchitectonic of alternating cell sparse (MZP, SR) and cell dense (SGL, DGL) layers. The distribution of the neuromarkers across the MZ also showed layer specificity. MAP2-positive cells were only found in the SGL. N200 and Reelin-positive neurons in the MZP. N200-positive processes were forming a plexus at the DGL level. All of the N200-posit

KW - Cajal–Retzius cells

KW - Cortical development

KW - Human fetal brain

KW - Marginal zone

KW - Neurofilaments

KW - Reelin

U2 - 10.3389/fnana.2016.00026

DO - 10.3389/fnana.2016.00026

M3 - Article

VL - 10

SP - 26

JO - Frontiers in Neuroanatomy

JF - Frontiers in Neuroanatomy

SN - 1662-5129

ER -